Identification of X-linked genes required for migration and programmed cell death of Drosophila melanogaster germ cells.
Identification of X-linked genes required for migration and programmed cell death of Drosophila melanogaster germ cells.
复制标题
鉴定果蝇生殖细胞迁移和程序性细胞死亡所需的 X 连锁基因。
DOI：
10.1093/genetics/162.1.273
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发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
Boswell,RobertE
中科院分区：
文献类型：
--
作者：
Coffman,ClarkR;Strohm,RachelC;Oakley,FredrickD;Yamada,Yukiko;Przychodzin,Danielle;Boswell,RobertE
果蝇的生殖细胞在胚胎的后极形成，并迁移到体细胞性腺。大约50%的形成的生殖细胞达到了它们的目标。胚胎内的游离细胞经历发育调节的细胞死亡。以前的研究已经确定了一些常染色体基因调控生殖细胞迁移，但控制生殖细胞死亡的基因是未知的。为了鉴定生殖细胞迁移和/或死亡所需的X连锁基因，我们对破坏这些过程的突变进行了筛选。在这里，我们报告了scattershot和outdoors的鉴定，这两个基因调节生殖细胞的程序性死亡。scattershotgene的定义是一个突变，破坏生殖细胞迁移和死亡的生殖细胞异位性腺。母体和合子的表达是必需的，但迁移和细胞死亡功能可以在遗传上解耦。野生型cattershot的合子表达挽救了生殖细胞的寻路，但不能恢复错误细胞的程序性死亡。该基因是合子所必需的。在未分化的胚胎中，适当数量的生殖细胞被整合到性腺中，但异位到性腺的生殖细胞仍然存在。
Drosophila germ cells form at the posterior pole of the embryo and migrate to the somatic gonad. Approximately 50% of the germ cells that form reach their target. The errant cells within the embryo undergo developmentally regulated cell death. Prior studies have identified some autosomal genes that regulate germ cell migration, but the genes that control germ cell death are not known. To identify X-linked genes required for germ cell migration and/or death, we performed a screen for mutations that disrupt these processes. Here we report the identification ofscattershotandoutsiders, two genes that regulate the programmed death of germ cells. Thescattershotgene is defined by a mutation that disrupts both germ cell migration and the death of germ cells ectopic to the gonad. Maternal and zygotic expression ofscattershotis required, but the migration and cell death functions can be genetically uncoupled. Zygotic expression of wild-typescattershotrescues germ cell pathfinding, but does not restore the programmed death of errant cells. Theoutsidersgene is required zygotically. Inoutsidersmutant embryos, the appropriate number of germ cells is incorporated into the gonad, but germ cells ectopic to the gonad persist.