Miniaturized fiber-coupled confocal fluorescence microscope with an electrowetting variable focus lens using no moving parts.
Miniaturized fiber-coupled confocal fluorescence microscope with an electrowetting variable focus lens using no moving parts.
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DOI：
10.1364/ol.40.002553
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发表时间：
2015-06-01
期刊：
影响因子：
3.6
通讯作者：
Gibson EA
中科院分区：
文献类型：
--
作者：
Ozbay BN;Losacco JT;Cormack R;Weir R;Bright VM;Gopinath JT;Restrepo D;Gibson EA
我们报告了一种微型、轻质光纤耦合共焦荧光显微镜，它结合了电润湿变焦镜头，可为全三维 (3D) 成像提供轴向扫描。横向扫描是通过将我们的设备通过相干成像光纤束耦合到激光扫描共焦显微镜来完成的。该设备的光学组件组合在定制的 3D 打印适配器中，组装重量 <2 g，可以安装在鼠标头上。共焦切片提供约 12 µm 的轴向分辨率和约 80 µm 的轴向扫描范围。横向视场为 300 µm，横向分辨率为 1.8 µm。我们通过对用绿色荧光蛋白（GFP）标记的小鼠神经元组织的固定切片和琼脂糖凝胶中的荧光珠样品进行成像来确定这些参数。为了证明完整组织成像的可行性，我们解析了表达黄色荧光蛋白（YFP）的离体小鼠嗅神经纤维的多个光学切片。
We report a miniature, lightweight fiber-coupled confocal fluorescence microscope that incorporates an electrowetting variable focus lens to provide axial scanning for full three-dimensional (3D) imaging. Lateral scanning is accomplished by coupling our device to a laser-scanning confocal microscope through a coherent imaging fiber-bundle. The optical components of the device are combined in a custom 3D-printed adapter with an assembled weight of <2 g that can be mounted onto the head of a mouse. Confocal sectioning provides an axial resolution of ~12 µm and an axial scan range of ~80 µm. The lateral field-of-view is 300 µm, and the lateral resolution is 1.8 µm. We determined these parameters by imaging fixed sections of mouse neuronal tissue labeled with green fluorescent protein (GFP) and fluorescent bead samples in agarose gel. To demonstrate viability for imaging intact tissue, we resolved multiple optical sections of ex vivo mouse olfactory nerve fibers expressing yellow fluorescent protein (YFP).