Effects of interleukin-2 on nitric oxide production in molluscan innate immunity
Effects of interleukin-2 on nitric oxide production in molluscan innate immunity
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发表时间：
2008-04
影响因子：
0.7
通讯作者：
R. Barcia;JI Ramos-Martinez
中科院分区：
文献类型：
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作者：
R. Barcia;JI Ramos-Martinez
摘要血细胞是软体动物中负责免疫的细胞。存在于血淋巴的可溶性部分中的细胞因子、生长因子等调节免疫应答。炎性细胞因子白细胞介素-2（IL-2）诱导一氧化氮（NO）的合成，负责氧化过程。在软体动物的血细胞中，IL-2的存在诱导高亲和力受体的三个亚基的合成，以及随后的信号通路的激活，其中cAMP依赖性蛋白激酶（PKA）起主要作用，PKC起次要作用。在IL-2的存在下，PKA通过组成性酶mc-NOS激活NO合成。在冬季，PKC对一种新的诱导型mw-NOS的作用导致IL-2诱导的NO合成增加。根据我们的研究，这组代谢反应解释了贻贝血细胞NO产生的季节性变化。然而，经过两年的研究，并与2002年11月的威望石油泄漏巧合，这种影响被取消了至少两年后的灾难。
Summary The hemocytes are the cells responsible for the immunity in molluscs. Cytokines, growth factors, etc, present in the soluble fraction of the hemolymph modulate the immune response. The inflammatory cytokine interleukin-2 (IL-2) induces the synthesis of nitric oxide (NO), responsible for oxidizing processes. In hemocytes of mollusks, the presence of IL-2 induces the synthesis of the three subunits of the receptor of high affinity, and the subsequent activation of the signaling pathway where the cAMP-dependent protein kinase (PKA) plays the main role, with a secondary role of PKC. In the presence of IL-2, PKA activates NO synthesis through the constitutive enzyme mc-NOS. In winter, the action of PKC on a novel inducible form mw-NOS leads to an increase of the IL-2-induced NO synthesis. According to our studies, this set of metabolic reactions explains the seasonal variations in NO production by hemocytes of Mytilus galloprovincialis. However, after two years of studies, and coinciding with the Prestige oil spill in November 2002, this effect was cancelled for at least the two years following the catastrophe.