Label-free colorimetric detection of biothiols utilizing SAM and unmodified Au nanoparticles
Label-free colorimetric detection of biothiols utilizing SAM and unmodified Au nanoparticles
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利用 SAM 和未修饰的金纳米粒子对生物硫醇进行无标记比色检测
DOI：
10.1016/j.bios.2015.01.062
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发表时间：
2015-06-15
影响因子：
12.6
通讯作者：
Qiu, Jian-Ding
中科院分区：
文献类型：
--
作者：
Li, Zhi-Jian;Zheng, Xiang-Juan;Qiu, Jian-Ding
本文报道了一种基于比色测定的灵敏且选择性的生物硫醇传感器。 S-腺苷-L-甲硫氨酸（SAM）可以通过静电相互作用诱导未修饰的金纳米粒子（AuNP）选择性聚集。在存在生物硫醇，例如谷胱甘肽（GSH）、同型半胱氨酸（Hcy）和半胱氨酸（Cys）的情况下，由于形成AuS键，AuNP更倾向于与生物硫醇的硫醇而不是SAM反应。因此，AuNPs从聚集状态转变为分散状态，AuNPs抗聚集过程中相应的颜色变化可用于通过紫外可见光谱或肉眼对生物硫醇进行定量筛选。在优化条件下，Cys在0.4-1.2μM范围内，GSH在0.2-0.9μM范围内，Hcys在0.6-3.0μM范围内具有良好的线性关系。该测定法对 GSH、Cys 和 Hcys 的检测限分别为 35.8 nM、21.7 nM 和 62.4 nM。该比色测定对生物硫醇具有快速操作（5 分钟内）、高选择性和灵敏度，且动态范围可调。 (C) 2015 Elsevier B.V. 保留所有权利。
Herein, a sensitive and selective sensor for biothiols based on colorimetric assay is reported. S-adenosyl-L-methionine (SAM) could induce the selective aggregation of unmodified gold nanoparticles (AuNPs) by electrostatic interaction. In the presence of biothiols, such as glutathione (GSH), homocysteine (Hcy), and cysteine (Cys), AuNPs prefer to react with thiols of biothiols rather than SAM due to the formation of AuS bond. Thus, the AuNPs turn from the aggregation to the dispersion state, and the corresponding color variation in the process of anti-aggregation of AuNPs can be used for the quantitative screening of biothiols through UV-vis spectroscopy or by the naked eye. Under optimized conditions, a good linear relationship in the range of 0.4-1.2 mu M is obtained for Cys, 0.2-0.9 mu M for GSH, and 0.6-3.0 mu M for Hcys. The detection limits of this assay for GSH, Cys and Hcys are 35.8 nM, 21.7 nM, and 62.4 nM, respectively. This colorimetric assay exhibits rapid operation (within 5 min), high selectivity and sensitivity towards biothiols with tunable dynamic ranges. (C) 2015 Elsevier B.V. All rights reserved.