The Carbamate Aldicarb Altered the Gut Microbiome, Metabolome, and Lipidome of C57BL/6J Mice
The Carbamate Aldicarb Altered the Gut Microbiome, Metabolome, and Lipidome of C57BL/6J Mice
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DOI：
10.1021/acs.chemrestox.8b00179
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发表时间：
2019-01-01
影响因子：
4.1
通讯作者：
Lu, Kun
中科院分区：
文献类型：
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作者：
Gao, Bei;Chi, Liang;Lu, Kun
肠道微生物群高度参与宿主生理的许多方面，从能量收集到应激反应，并可以给宿主带来许多好处。肠道微生物群的发育可能受到遗传和环境因素的影响，包括杀虫剂。氨基甲酸酯类杀虫剂涕灭威在农业中广泛使用，引起了严重的公共卫生问题。然而，涕灭威对肠道微生物组、宿主代谢组和脂质组的影响尚未得到很好的研究。在此，我们使用多组学方法，包括16S rRNA测序，霰弹枪宏基因组测序，代谢组学和脂质组学，来阐明乙醇威诱导的肠道微生物组毒性和宿主代谢稳态。我们证明了涕灭威扰乱了肠道微生物群的发育轨迹，增强了肠道细菌的致病性，改变了复杂的脂质谱，并诱导了氧化应激、蛋白质降解和DNA损伤。脑代谢也受到了涕灭威的干扰。这些发现可能为氨基甲酸酯类杀虫剂的毒性提供新的认识。
The gut microbiome is highly involved in numerous aspects of host physiology, from energy harvest to stress response, and can confer many benefits to the host. The gut microbiome development could be affected by genetic and environmental factors, including pesticides. The carbamate insecticide aldicarb has been extensively used in agriculture, which raises serious public health concerns. However, the impact of aldicarb on the gut microbiome, host metabolome, and lipidome has not been well studied yet. Herein, we use multiomics approaches, including 16S rRNA sequencing, shotgun metagenomics sequencing, metabolomics, and lipidomics, to elucidate aldicarb-induced toxicity in the gut microbiome and the host metabolic homeostasis. We demonstrated that aldicarb perturbed the gut microbiome development trajectory, enhanced gut bacterial pathogenicity, altered complex lipid profile, and induced oxidative stress, protein degradation, and DNA damage. The brain metabolism was also disturbed by the aldicarb exposure. These findings may provide a novel understanding of the toxicity of carbamate insecticides.