HYPOXANTHINE AND XANTHINE LEVELS DETERMINED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN PLASMA, ERYTHROCYTE, AND URINE SAMPLES FROM HEALTHY-SUBJECTS - THE PROBLEM OF HYPOXANTHINE LEVEL EVOLUTION AS A FUNCTION OF TIME
HYPOXANTHINE AND XANTHINE LEVELS DETERMINED BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY IN PLASMA, ERYTHROCYTE, AND URINE SAMPLES FROM HEALTHY-SUBJECTS - THE PROBLEM OF HYPOXANTHINE LEVEL EVOLUTION AS A FUNCTION OF TIME
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DOI：
10.1016/0003-2697(83)90568-7
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发表时间：
1983-01-01
影响因子：
2.9
通讯作者：
GONNET, C
中科院分区：
文献类型：
--
作者：
BOULIEU, R;BORY, C;GONNET, C
通过反相高效液相色谱法（HPLC）测定血浆、红细胞和尿液样本中的次黄嘌呤和黄嘌呤水平。当全血在采样和离心之间储存在室温下时，红细胞和血浆样品中的次黄嘌呤浓度增加。此外，当红细胞样品在分析前在室温下分开时，红细胞样品中的次黄嘌呤浓度增加，而血浆次黄嘌呤水平保持恒定。该结果证明了在室温下，随着时间的推移，红细胞中内源性形成次黄嘌呤。这些研究表明，血液样本的采集、运输和处理需要严格的条件。为了获得准确的结果，血液采集后必须立即离心。红细胞和血浆样品必须冷冻储存，直至进行脱蛋白和HPLC分析。在这些条件下，次黄嘌呤和黄嘌呤在血浆中的浓度为2.5 ± 0.5。1和1.4 .+-。0.7μ M。在红细胞样品中，次黄嘌呤浓度达到8.0 ±。6.2μ M。
The levels of hypoxanthine and xanthine are determined in plasma, erythrocyte and urine samples by a reverse-phase high-performance liquid chromatographic (HPLC) method. The hypoxanthine concentration increases in erythrocyte and plasma samples when whole blood is stored at room temperature between sampling and centrifugation. Furthermore, the hypoxanthine concentration increases in erythrocyte samples when they are kept apart at a room temperature before analysis, whereas the plasma hypoxanthine level remains constant. This result proves an endogenous formation of hypoxanthine in erythrocytes with time, at room temperture. These studies show the necessity of rigorous conditions for the collection, transport, and treatment of blood samples. To achieve accurate results, the blood must be centrifuged immediately after collection. The erythrocyte and plasma samples must be stored frozen until deproteinization and HPLC analysis. Under these conditions, the concentrations of hypoxanthine and xanthine in plasma are 2.5 .+-. 1 and 1.4 .+-. 0.7 .mu.M, respectively. In erythrocyte samples, hypoxanthine concentration reaches 8.0 .+-. 6.2 .mu.M.