SALT STRESS IS AN ENVIRONMENTAL SIGNAL AFFECTING DEGRADATIVE ENZYME-SYNTHESIS IN BACILLUS-SUBTILIS
SALT STRESS IS AN ENVIRONMENTAL SIGNAL AFFECTING DEGRADATIVE ENZYME-SYNTHESIS IN BACILLUS-SUBTILIS
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DOI：
10.1128/jb.177.9.2403-2407.1995
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发表时间：
1995-05-01
影响因子：
3.2
通讯作者：
RAPOPORT, G
中科院分区：
文献类型：
--
作者：
KUNST, F;RAPOPORT, G
盐胁迫对枯草芽孢杆菌降解酶的合成有重要影响。盐胁迫强烈刺激sacB的表达，编码果聚糖蔗糖酶（约9倍），并下调aprE的表达，编码碱性蛋白酶（约6倍）。这表明DegS-DegU双组分系统参与了盐胁迫的传感。此外，已经表明sacB的表达水平强烈依赖于生长条件;其表达水平在琼脂平板上生长的细胞中比在液体培养基中生长的细胞高约8倍。
Growth under conditions of salt stress has important effects on the synthesis of degradative enzymes in Bacillus subtilis. Salt stress strongly stimulates the expression of sacB, encoding levansucrase (about ninefold), and downregulates the expression of aprE, encoding alkaline protease (about sixfold). It is suggested that the DegS-DegU two-component system is involved in sensing salt stress. Moreover, it has been shown that the level of sacB expression strongly depends on the growth conditions; its expression level is about eightfold higher in cells grown on agar plates than in cells grown in liquid medium.