Popeye domain containing gene 2 (Popdc2) is a myocyte-specific differentiation marker during chick heart development
Popeye domain containing gene 2 (Popdc2) is a myocyte-specific differentiation marker during chick heart development
复制标题
DOI：
10.1002/dvdy.20015
复制
发表时间：
2004-03-01
影响因子：
2.5
通讯作者：
Brand, T
中科院分区：
文献类型：
--
作者：
Breher, SS;Mavridou, E;Brand, T
Popeye domain containing（popdc）基因是一个编码肌细胞质膜蛋白的新基因家族，具有三个N端跨膜结构域和一个胞质羧基端。在脊椎动物中，已经描述了Popdc基因家族的三个成员。然而，在鸡系统中，只有两个cDNA，Popdc 1和Popdc 3，以前已被克隆。通过对鸡表达序列标签数据库的筛选，我们鉴定了5个羧基末端不同的选择性剪接的鸡Popdc 2 cDNA。北方印迹分析显示Popdc 2主要在心肌中表达，在骨骼肌中表达较弱。通过整体原位杂交，首次在Hamburger和汉密尔顿（HH）7期心脏区域的前部检测到鸡Popdc 2。在管状心脏中，心房和心室前体细胞对Popdc 2呈阳性染色。在流出道和静脉窦的心肌中观察到较弱的表达。到HH第18期，外弯心肌被强烈染色，而内弯心肌中的表达可忽略不计。Popdc 2表达不存在于心内膜和心外膜器官。在HH阶段36，Popdc 2表达局限于致密层心肌。除心脏外，还在肌节和四肢的肌肉形成区域中观察到Popdc 2表达。我们的研究结果表明，Popdc 2是高表达的发育中的心脏，并可能作为一个新的标记心肌分化的鸡胚。发展动力学229.695-702，2004年。(C)2004 Wiley-Liss，Inc.
The Popeye domain containing (popdc) genes constitute a novel gene family encoding proteins of the plasma membrane in muscle cells, with three N-terminal transmembrane domains and a cytoplasmic carboxy terminus. In vertebrates, three members of the Popdc gene family have been described. However, in the chick system only two cDNAs, Popdc1 and Popdc3, have been cloned previously. By screening a chick expressed sequence tag database, we report here the identification of five alternatively spliced chick Popdc2 cDNAs with different carboxy termini. Northern blot analysis revealed expression of Popdc2 predominantly in the myocardium and weaker expression in skeletal muscle. By whole-mount in situ hybridization, chick Popdc2 was first detected at Hamburger and Hamilton (HH) stage 7 within the anterior part of the heart fields. In the tubular heart, atrial and ventricular precursor cells stained positively for Popdc2. Weaker expression was observed in myocardium of the outflow tract and sinus venosus. By HH stage 18, the outer curvature myocardium was strongly stained, whereas expression in myocardium of the inner curvature was negligible. Popdc2 expression was absent from the endocardium and propepicardial organ. At HH stage 36, Popdc2 expression was confined to the compact layer myocardium. In addition to the heart, Popdc2 expression was also observed in the myotome and in the muscle-forming fields of the limbs. Our results indicate that Popdc2 is highly expressed in the developing heart and may serve as a novel marker of myocardial differentiation in the chick embryo. Developmental Dynamics 229.695-702, 2004. (C) 2004 Wiley-Liss, Inc.