In vivo confocal microscopy in fungal keratitis
In vivo confocal microscopy in fungal keratitis
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DOI：
10.1136/bjo.2006.107243
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发表时间：
2007-05-01
影响因子：
4.1
通讯作者：
Baudouin, Christophe
中科院分区：
文献类型：
--
作者：
Brasnu, Emmanuelle;Bourcier, Tristan;Baudouin, Christophe
背景：真菌性角膜炎是一种常见的致盲性眼病，在世界范围内，尤其是在发展中国家。鉴于最近由于接触透镜溶液导致接触透镜佩戴者中镰刀菌角膜炎感染的增加，食品和药物管理局最近发布了警告，使其成为发达国家的公共卫生问题。目的：显示使用海德堡视网膜断层扫描仪II-罗斯托克角膜模块的体内共焦显微镜成像的优点方法：对5例真菌性角膜炎患者和3例受镰刀菌、烟曲霉菌和白色念珠菌污染的供体角膜进行HRTII-RCM共聚焦显微镜检查。角膜涂片和培养的直接显微镜评价显示，在4例和1例白色念珠菌的存在下，F solani。HRTII-RCM检查感染的患者和污染的供体角膜发现了许多高对比度的元素，类似镰刀菌，曲霉菌菌丝或念珠菌pseudofilaments在前stroma.Conclusion：HRTII-RCM在体内共聚焦显微镜是一种新的，非侵入性和快速的技术，用于真菌性角膜炎的早期诊断，显示高分辨率的图像，类似于真菌的结构，在疾病的早期阶段。
Background: Fungal keratitis is a major blinding eye disease found throughout the world, particularly in developing countries. Given the recent increase in Fusarium keratitis infections in contact lens wearers owing to contact lens solutions, a warning was recently issued by the Food and Drug Administration, making it a public health concern in developed countries.Objective: To show the advantages of in vivo confocal microscopy imaging using the Heidelberg Retina Tomograph II-Rostock Cornea Module (HRTII-RCM) in the early diagnosis of fungal keratitis.Methods: HRTII-RCM confocal microscopy was performed on five patients presenting with fungal keratitis and on three donor corneas contaminated with Fusarium solani, Aspergillus fumigatus and Candida albicans.Results: Direct microscopic evaluation of corneal smears and culture revealed the presence of F solani in four cases and C albicans in one case. HRTII-RCM examination of the infected patients and contaminated donor corneas revealed numerous high-contrast elements resembling Fusarium, Aspergillus hyphae or Candida pseudofilaments in the anterior stroma.Conclusion: HRTII-RCM in vivo confocal microscopy is a new, non-invasive and rapid technique for the early diagnosis of fungal keratitis, showing high-resolution images resembling fungal structures in the early phase of the disease.