Native vascular related NAC transcription factors are efficient regulator of multiple classes of secondary wall associated genes in banana
Native vascular related NAC transcription factors are efficient regulator of multiple classes of secondary wall associated genes in banana
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DOI：
10.1016/j.plantsci.2017.09.018
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发表时间：
2017-12-01
期刊：
影响因子：
5.2
通讯作者：
Ganapathi, T. R.
中科院分区：
文献类型：
--
作者：
Negi, Sanjana;Tak, Himanshu;Ganapathi, T. R.
木质部导管元件的次生壁沉积受血管相关NAC转录因子（VNDs）的调控。我们发现香蕉的三个vnd1 （MusaVND1， MusaVND2和MusaVND3）通过结合其5'上游调控区直接调控多个次级壁相关基因。含有P-MusaVND1:GUS、P-MusaVND2:GUS或P-MusaVND3:GUS的转基因香蕉在木质化组织中显示特异性GUS染色。MusaVND1、MusaVND2和MusaVND3编码转录激活因子作为其c端区驱动酵母体内报告基因的表达。凝胶移位实验纯化的MusaVND1、MusaVND2和MusaVND3蛋白与19bp的NAC第二壁结合元件（SNBE）结合，而不能与突变的SNBE结合。在香蕉与程序性细胞死亡（XCP1）、细胞壁修饰（IRXI/CesA8、IRX3/CesA7、IRX5/CesA4、IRX8、IRX10和IRX12）和转录调控（MYB52、MYB48/59、MYB85、MYB58/72、MYB46和MYB83）相关的重要次级壁相关基因的5′上游调控区域中发现了推测的SNBE位点，这些调控区域的迁移被MusaVND1、MusaVND2和MusaVND3阻滞。在过表达MusaVND1、MusaVND2或MusaVND3的转基因香蕉中，这些重要的次生壁相关基因的转录水平升高。目前的研究表明，启动子在香蕉（一种潜在的生物燃料作物）的壁修饰中具有潜在的应用前景，并提示了植物次生壁沉积的复杂转录调控。
Secondary-wall deposition in xylem vessel elements is regulated by vascular-related NAC transcription factors (VNDs). We show that three banana VNDs (MusaVND1, MusaVND2 and MusaVND3) directly regulate multiple secondary-wall associated genes by binding to their 5'-upstream regulatory region. Transgenic banana harboring either P-MusaVND1:GUS, P-MusaVND2:GUS or P-MusaVND3:GUS showed specific GUS staining in lignified tissues. MusaVND1, MusaVND2 and MusaVND3 encodes transcriptional-activators as its C-terminal region drive expression of reporter genes in vivo in yeast. Purified MusaVND1, MusaVND2 and MusaVND3 proteins in gel shift assay bind to 19-bp secondary-wall NAC binding element (SNBE) while it fails to bind mutated SNBE. Putative SNBE sites in the 5'-upstream regulatory region of important secondary-wall associated genes related to programmed cell death (XCP1), cell-wall modification (IRXI/CesA8, IRX3/CesA7,IRX5/CesA4, IRX8, IRX10 and IRX12) and transcriptional regulation (MYB52, MYB48/59, MYB85, MYB58/72, MYB46, and MYB83) in banana was identified and mobility of these regulatory regions got retarded by MusaVND1, MusaVND2 and MusaVND3. Transcript level of these important secondary wall associated genes were elevated in transgenic banana over expressing either MusaVND1, MusaVND2 or MusaVND3. Present study suggested promoters with prospective utilization in wall modification in banana (a potential biofuel crop) and suggest a complex transcriptional regulation of secondary wall deposition in plants.