Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet
Relative bioavailabilities of organic zinc sources with different chelation strengths for broilers fed a conventional corn-soybean meal diet
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DOI：
10.2527/jas.2008-1212
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发表时间：
2009-06-01
影响因子：
3.3
通讯作者：
Liu, B.
中科院分区：
文献类型：
--
作者：
Huang, Y. L.;Lu, L.;Liu, B.
以硫酸锌为对照，研究了3种不同螯合强度有机锌源中锌的相对生物利用率。将1，092只1日龄雄性肉仔鸡随机分配到6个重复笼中（每笼14只鸡），共13个处理。日粮处理包括基础玉米-豆粕日粮（27.82 mg Zn/kg DM）补充0、30、60或90 mg添加的Zn作为试剂ZnSO 4，或Qf为6.5的Zn源（11.93%Zn; Zn AA C）、30.7（13.27%Zn; Zn Pro B）或944.0（18.61%Zn; Zn Pro A）/kg，其分别被认为是弱、中等或强Qf。分别于6、10、14日龄测定骨锌、胰腺锌、胰腺金属硫蛋白（MT）浓度和胰腺MT信使RNA（mRNA）。结果表明，随着日粮锌水平的提高，各日龄肉仔鸡的骨锌、胰腺锌、胰腺MT含量和胰腺MT mRNA均呈线性增加（P < 0.001）。6日龄时，胰腺MT mRNA在不同锌源间差异极显著（P < 0.001），10日龄（P = 0.08）和14日龄（P = 0.06）时差异极显著（P <0.01）。对于所有响应标准，线性模型的R-2在第6天大于第10天或第14天。根据胰腺MT mRNA浓度与日粮锌摄入量的多元线性回归斜率比，6日龄时Zn AA C、Zn Pro B和Zn Pro A相对于硫酸锌（100%）的生物利用率分别为100.0、121.1和72.3%。结果表明，胰腺MT mRNA含量比胰腺MT含量等指标更能敏感地反映有机锌源的生物有效性差异。有机锌源的生物有效性与其Q（f）密切相关。
An experiment was conducted to estimate relative bioavailability of Zn in 3 organic zinc sources with different chelation strength (Q(f)) compared with ZnSO4. A total of 1,092, 1-d-old male broiler chicks were assigned randomly to 6 replicate cages (14 chicks per cage) for each of 13 treatments. Dietary treatments included the basal corn-soybean meal diet (27.82 mg of Zn/kg of DM) supplemented with 0, 30, 60, or 90 mg of added Zn as reagent ZnSO4, or Zn sources with Qf of 6.5 (11.93% Zn; Zn AA C), 30.7 (13.27% Zn; Zn Pro B), or 944.0 (18.61% Zn; Zn Pro A)/kg, which are considered as weak, moderate, or strong Qf, respectively. Bone Zn, pancreas Zn, pancreas metallothionein (MT) concentration, and pancreas MT messenger RNA (mRNA) were analyzed at 6, 10, or 14 d of age. The results showed that bone Zn, pancreas Zn, pancreas MT concentration, and pancreas MT mRNA increased linearly (P < 0.001) as dietary Zn concentration increased at all ages. At 6 d of age, pancreas MT mRNA differed (P < 0.001) among dietary Zn sources, and the same tendency was observed at 10 (P = 0.08) or 14 d (P = 0.06) of age. The R-2 for a linear model was greater on d 6 than d 10 or 14 for all the response criteria. Based on slope ratios from the multiple linear regression of pancreas MT mRNA concentration on daily intake of dietary Zn, the bioavailability of Zn AA C, Zn Pro B, and Zn Pro A relative to ZnSO4 (100%) were 100.0, 121.1, and 72.3%, respectively, at 6 d of age. The results indicated that MT mRNA concentration in pancreas was more sensitive in reflecting differences in bioavailability among organic Zn sources than the MT concentration in pancreas or other indices. Moreover, the bioavailability of organic Zn sources was closely related to their Q(f).