A cotton NAC domain transcription factor, GhFSN5, negatively regulates secondary cell wall biosynthesis and anther development in transgenic Arabidopsis
A cotton NAC domain transcription factor, GhFSN5, negatively regulates secondary cell wall biosynthesis and anther development in transgenic Arabidopsis
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棉花 NAC 结构域转录因子 GhFSN5 负向调节转基因拟南芥次生细胞壁生物合成和花药发育
DOI：
10.1016/j.plaphy.2019.11.030
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发表时间：
2020
影响因子：
6.5
通讯作者：
Xu Wenliang
中科院分区：
文献类型：
--
作者：
Sun Qianwen;Huang Junfeng;Guo Yifan;Yang Mingming;Guo Yanjun;Li Juan;Zhang Jie;Xu Wenliang
NAC结构域转录因子（NAC domain transcription factors，TFs）是植物特异性转录调控因子，其中一些在植物次生细胞壁（secondary cell wall，SCW）生物合成中起重要作用。棉花是最重要的天然纤维生产作物之一，其成熟纤维SCW含有90%以上的纤维素和极少量的木聚糖和木质素，但对纤维SCW形成的分子机制知之甚少。我们以前确定了七个纤维优先表达NAC成员，GhFSN 1 -7。其中一个，GhFSN 1，被证明是积极调节纤维SCW增厚，但其他GhFSN成员的功能仍然未知。在这项研究中，GhFSN 5的作用进行了解剖。qRT-PCR分析表明GhFSN 5主要在纤维SCW增厚期转录。此外，GhFSN 5还与大量的纤维SCW生物合成基因和SCW相关转录因子共表达。GhFSN 5在拟南芥中的异源表达导致植株角果变小，严重不育。转基因株系的花药开裂没有受到实质性的影响，但大多数花粉塌陷，不能存活。此外，与野生型相比，转基因株系花序茎和根中的纤维素和木质素含量降低。此外，一组纤维素，木聚糖和木质素的SCW生物合成基因和参与调控SCW形成的转录因子在转基因植物中下调。我们的研究结果表明，GhFSN 5作为一个负调控的SCW形成和花药发育，扩大了我们的理解转录调控SCW生物合成。
NAC domain transcription factors (TFs) are plant-specific transcriptional regulators, some of which play crucial roles in secondary cell wall (SCW) biosynthesis in plants. Cotton is one of the most important natural fiber producing crops, whose mature fiber SCW contains more than 90% cellulose with very small amounts of xylan and lignin, but little is known about the molecular mechanism underlying fiber SCW formation. We previously identified seven fiber preferentially expressed NAC members, GhFSN1-7. One, GhFSN1, was demonstrated to positively regulate fiber SCW thickening, but the functions of other GhFSN members remain unknown. In this study, roles of GhFSN5 were dissected. qRT-PCR analysis showed thatGhFSN5was predominantly transcribed during the fiber SCW thickening stage. In addition, a large number of fiber SCW biosynthetic genes and SCW-related TFs were co-expressed withGhFSN5. Heterologous expression ofGhFSN5in Arabidopsis resulted in plants with smaller siliques and severe sterility. Anther dehiscence in transgenic lines was not substantially affected, but most pollen was collapsed and nonviable. Furthermore, cellulose and lignin contents in inflorescence stems as well as roots were reduced in transgenic lines, compared with the wild type. Moreover, a set of SCW biosynthetic genes for cellulose, xylan and lignin and several transcription factors involved in regulation of SCW formation were down-regulated in transgenic plants. Our findings indicate that GhFSN5 acts as a negative regulator of SCW formation and anther development and expands our understanding of transcriptional regulation of SCW biosynthesis.