PURIFICATION AND PHYSICAL CHARACTERIZATION OF TYROSYL RIBONUCLEIC ACID SYNTHETASES FROM ESCHERICHIA COLI AND BACILLUS SUBTILIS
PURIFICATION AND PHYSICAL CHARACTERIZATION OF TYROSYL RIBONUCLEIC ACID SYNTHETASES FROM ESCHERICHIA COLI AND BACILLUS SUBTILIS
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DOI：
10.1021/bi00869a033
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发表时间：
1966-01-01
期刊：
影响因子：
2.9
通讯作者：
BERG, P
中科院分区：
文献类型：
--
作者：
CALENDAR, R;BERG, P
E. coli和B.枯草芽孢杆菌已被纯化至接近同质。这些酶的分子量分别为95，000和88，000。它们可以在淀粉或聚丙烯酰胺凝胶上电泳分离。急诊大肠杆菌酶含有14个半胱氨酸残基/摩尔，而B.枯草杆菌酶的活性为2-3。蚂蚁血清到E.大肠杆菌酶中和并沉淀大肠杆菌。大肠杆菌酶，但不与B交叉反应。枯草杆菌酶抗B抗体。枯草芽孢杆菌酶中和，也显示出部分交叉反应与E.大肠杆菌酶。
Tyrosyl ribonucleic-acid synthetases from E. coli and B. subtilis have been purified to near homogeneity. These enzymes have molecular weights of 95,000 and 88,000, respectively. They can be separated electrophoretically on starch or polyacrylamide gels. The E. coli enzyme contains 14 half-cystine residues/mole while the B. subtilis enzyme has 2-3. Antlserum to the E. coli enzyme neutralizes and precipitates the E. coli enzyme but does not cross-react with the B. subtilis enzyme. Antiserum to the B. subtilis enzyme neutralizes and also shows partial cross-reaction with the E. coli enzyme.