A Protocol for Collecting Human Cardiac Tissue for Research.
A Protocol for Collecting Human Cardiac Tissue for Research.
复制标题
DOI：
10.13023/vad.2016.12
复制
发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Campbell KS
中科院分区：
文献类型：
--
作者：
Blair CA;Haynes P;Campbell SG;Chung C;Mitov MI;Dennis D;Bonnell MR;Hoopes CW;Guglin M;Campbell KS
这份手稿描述了肯塔基大学的一项协议，该协议允许转译研究团队收集可用于生物研究的人体心肌。自2008年开始这项议定书以来，我们已经获得了大量的实践经验，我们希望其他团体或许能够从我们的努力中学习。到目前为止，我们已经从230名患者那里获得了4000份样本。我们收集的组织来自器官捐赠者和因心力衰竭而接受心脏移植或心脏辅助装置的患者。我们的手稿一开始就描述了人体样本在心脏研究中的重要性。随后，我们描述了获得患者同意的过程，运行该方案的成本，以及我们遇到的一些问题和实际困难。最后，我们为其他可能考虑启动类似方案的研究人员提供了一些建议。
This manuscript describes a protocol at the University of Kentucky that allows a translational research team to collect human myocardium that can be used for biological research. We have gained a great deal of practical experience since we started this protocol in 2008, and we hope that other groups might be able to learn from our endeavors. To date, we have procured ~4000 samples from ~230 patients. The tissue that we collect comes from organ donors and from patients who are receiving a heart transplant or a ventricular assist device because they have heart failure. We begin our manuscript by describing the importance of human samples in cardiac research. Subsequently, we describe the process for obtaining consent from patients, the cost of running the protocol, and some of the issues and practical difficulties that we have encountered. We conclude with some suggestions for other researchers who may be considering starting a similar protocol.