Role of tissue-specific blood flow and tissue recruitment in insulin-mediated glucose uptake of human skeletal muscle
Role of tissue-specific blood flow and tissue recruitment in insulin-mediated glucose uptake of human skeletal muscle
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DOI：
10.1161/01.cir.98.3.234
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发表时间：
1998-07-21
期刊：
影响因子：
37.8
通讯作者：
Cobelli, C
中科院分区：
文献类型：
--
作者：
Bonadonna, RC;Saccomani, MP;Cobelli, C
背景-关于胰岛素诱导的血管舒张是否在肢体葡萄糖摄取的调节中起机械作用，存在明确的证据。可以预测，如果胰岛素增加血流量，造成组织招聘，这种机制将提高肢体葡萄糖uptake.Methods和Results-Twenty健康受试者进行了研究，前臂灌注技术结合正常血糖胰岛素钳夹技术。10名受试者在生理胰岛素浓度（约400 pmol/L）下进行研究，另外10名受试者在超生理胰岛素浓度（约5600 pmol/L）下进行研究。另外4例受试者接受了生理盐水对照研究。将不可代谢的细胞外标记物（1-[H-3]-L-葡萄糖）脉冲注射到肱动脉中，并在同侧前臂深静脉中测量其洗脱曲线，并用于估计细胞外分布容积，从而估计前臂深静脉引流的肌肉组织量。在生理盐水输注和生理水平的高胰岛素血症期间，我们观察到前臂深静脉引流的血流和/或肌肉组织没有变化。然而，超生理性高胰岛素血症加速了前臂总血流量（45.0 ± 1.8 vs 36.5 ± 1.3 mL.min（-1）.kg（-1），P
Background-Conflicting evidence exist concerning whether insulin-induced vasodilation plays a mechanistic role in the regulation of limb glucose uptake. It can be predicted that if insulin augments blood flow by causing tissue recruitment, this mechanism would enhance limb glucose uptake.Methods and Results-Twenty healthy subjects were studied with the forearm perfusion technique in combination with the euglycemic insulin clamp technique. Ten subjects were studied at physiological insulin concentrations (approximate to 400 pmol/L) and the other 10 at supraphysiological insulin concentrations (approximate to 5600 pmoI/L). Four additional subjects underwent a saline control study. Pulse injections of a nonmetabolizable extracellular marker (1-[H-3]-L-glucose) were administered into the brachial artery, and its washout curves were measured in one ipsilateral deep forearm Vein and used to estimate the extracellular volume of distribution and hence the amount of muscle tissue drained by the deep forearm vein. Both during saline infusion and at physiological levels of hyperinsulinemia we observed no changes in blood flow and/or muscle tissue drained by the deep forearm vein. However, supraphysiological hyperinsulinemia accelerated total forearm blood flow (45.0+/-1.8 versus 36.5+/-1.3 mL.min(-1).kg(-1), P