Epigenetic reprogramming of embryos derived from sperm frozen at-20A°C
Epigenetic reprogramming of embryos derived from sperm frozen at-20A°C
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DOI：
10.1007/s11427-012-4309-8
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发表时间：
2012-04-01
影响因子：
9.1
通讯作者：
Gao GuoLan
中科院分区：
文献类型：
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作者：
Chao ShiBin;Li JianChun;Gao GuoLan
精子冷冻保存是一种用于保存对生物医学研究有价值的动物物种和动物品系精子的策略。非常需要一种在应用胞浆内单精子注射（ICSI）后保存精子的简单方法。此前已有研究表明，-20A 摄氏度下冷冻的精子可以激活卵母细胞并支持足月胚胎发育。然而，表观遗传重编程可能会受到环境和配子操纵的影响。在这里，我们研究了胚胎表观遗传重编程，包括 DNA 甲基化和组蛋白修饰，来自在 -20A 摄氏度下保存且不含冷冻保护剂的精子的胚胎。结果表明，尽管受精和胚胎发育能力均下降，但冷冻精子胚胎的动态表观遗传重编程与新鲜精子胚胎的重编程相似。本研究报告的结果表明，不含冷冻保护剂的冷冻精子对于 ICSI 来说在表观遗传学上是安全的。
Cryopreservation of spermatozoa is a strategy that has been used to conserve the sperm of animal species and animal strains that are valuable for biomedical research. A simple method for preserving spermatozoa after application of intracytoplasmic sperm injection (ICSI) is much needed. It has been shown previously that spermatozoa frozen at -20A degrees C can activate oocytes and support full-term embryo development. However, epigenetic reprogramming could be affected by the environment and by the manipulation of gametes. Here, we investigated embryo epigenetic reprogramming including DNA methylation and histone modification, in embryos derived from sperm preserved at -20A degrees C without cryoprotectants. The results showed that although both fertilization and embryo developmental competence were decreased, the dynamic epigenetic reprogramming of embryos derived from frozen sperm was similar to the reprogramming of embryos derived from fresh sperm. The results reported in this study indicate that sperm frozen without cryoprotectant is epigenetically safe for ICSI.