Development of pigment-dispersing hormone-immunoreactive neurons in the American lobster: homology to the insect circadian pacemaker system?
Development of pigment-dispersing hormone-immunoreactive neurons in the American lobster: homology to the insect circadian pacemaker system?
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DOI：
10.1007/s00441-008-0728-z
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发表时间：
2009-02-01
影响因子：
3.6
通讯作者：
Beltz, Barbara S.
中科院分区：
文献类型：
--
作者：
Harzsch, Steffen;Dircksen, Heinrich;Beltz, Barbara S.
我们已经研究了色素分散激素（PDH）的免疫反应神经元的胚胎中的美国龙虾Homarus americanus米尔恩爱德华兹，1837年（十足目，Reptantia，Homarida），通过使用抗血清对β-PDH。这种肽在眼柄和前脑的终髓中是可检测的，早在胚胎发育的20%时，PDH免疫反应就存在于其中。在个体发育过程中，一个精心设计的系统PDH免疫反应神经元和纤维的眼柄和proocerebrum的发展，而较少的标签是存在于deuto和tritocerebrum和腹神经索。窦腺在孵化时由PDH神经突支配。这种模式的PDH免疫反应性已与在各种昆虫物种中发现的。髓质中对色素分散因子免疫反应的神经元已被证明是产生昆虫昼夜节律的系统的中心组成部分。我们的研究结果表明，鉴于神经元胞体的位置和突起的投射模式，免疫标记的昆虫髓质神经元在甲壳动物眼柄中具有同源对应物。由于运动和其他活动在甲壳类动物遵循不同的昼夜节律与昆虫中观察到的，我们建议，PDH免疫反应阳性的髓质神经元在甲壳类动物参与这些节奏的产生。
We have examined the development of pigment-dispersing hormone (PDH)-immunoreactive neurons in embryos of the American lobster Homarus americanus Milne Edwards, 1837 (Decapoda, Reptantia, Homarida) by using an antiserum against beta-PDH. This peptide is detectable in the terminal medulla of the eyestalks and the protocerebrum where PDH immunoreactivity is present as early as 20% of embryonic development. During ontogenesis, an elaborate system of PDH-immunoreactive neurons and fibres develops in the eyestalks and the protocerebrum, whereas less labelling is present in the deuto- and tritocerebrum and the ventral nerve cord. The sinus gland is innervated by PDH neurites at hatching. This pattern of PDH immunoreactivity has been compared with that found in various insect species. Neurons immunoreactive to pigment-dispersing factor in the medulla have been shown to be a central component of the system that generates the circadian rhythm in insects. Our results indicate that, in view of the position of the neuronal somata and projection patterns of their neurites, the immunolabelled medulla neurons in insects have homologous counterparts in the crustacean eyestalk. Since locomotory and other activities in crustaceans follow distinct circadian rhythms comparable with those observed in insects, we suggest that PDH-immunoreactive medulla neurons in crustaceans are involved in the generation of these rhythms.