Evaluation of Islet Purification Methods for Making a Continuous Density Gradient and Loading Tissue
Evaluation of Islet Purification Methods for Making a Continuous Density Gradient and Loading Tissue
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DOI：
10.1177/2155179017733090
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发表时间：
2018-05-29
期刊：
影响因子：
--
通讯作者：
Noguchi, Hirofumi
中科院分区：
文献类型：
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作者：
Ebi, Nana;Miyagi-Shiohira, Chika;Noguchi, Hirofumi
胰岛纯化是胰岛移植中胰岛分离的最重要步骤之一。我们先前报道了使用大塑料瓶的纯化方法有效地从猪胰腺中获得了高产量的胰岛。在这项研究中，我们评估了制作连续密度梯度和加载组织的方法。一种方法涉及将消化的组织装载在连续密度梯度的顶部（顶部装载）。另一种方法是将消化的组织与低密度溶液混合，然后形成连续梯度（混合加载）。两种纯化方法在胰岛产量、存活率或纯度、评分或纯化后的刺激指数方面无显著差异。此外，在移植后normoflavin的可获得性或适用性方面没有显著差异。我们的研究显示了这两种胰岛纯化方法的等效性。
Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from porcine pancreas. In this study, we evaluated the methods for making a continuous density gradient and loading tissue. One method involved loading digested tissue on top of a continuous density gradient (top loading). The other method involved mixing digested tissue with low-density solution and then making a continuous gradient (mixed loading). There were no significant differences between the 2 purification methods in terms of the islet yield, rate of viability or purity, score, or in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of posttransplantation normoglycemia. Our study shows the equivalency of these 2 methods of islet purification.