A COMPARATIVE-STUDY OF NEUTROPHIL PURIFICATION AND FUNCTION
A COMPARATIVE-STUDY OF NEUTROPHIL PURIFICATION AND FUNCTION
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DOI：
10.1016/0022-1759(85)90363-1
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发表时间：
1985-01-01
影响因子：
2.2
通讯作者：
JONES, HP
中科院分区：
文献类型：
--
作者：
GRISHAM, MB;ENGERSON, TD;JONES, HP
目前许多实验室使用几种不同的方法分离人中性粒细胞。这些方法包括通过葡聚糖沉降随后水裂解的部分纯化，以及利用与葡聚糖沉降偶联的不连续密度梯度和在某些情况下低渗裂解的更完整的纯化程序。一些研究者避免使用某些纯化方案，因为特定方法中使用的某些步骤或试剂可能会对他们希望测量的中性粒细胞的功能参数产生不利影响。尽管存在这些担忧，但尚未对通过各种方法制备的中性粒细胞的功能状态进行系统比较。本研究比较了4种常用的中性粒细胞分离方法。本研究的结果表明，虽然通过分离程序测定了中性粒细胞的产率和纯度，但所有细胞在趋化性能、去脂能力以及产生超氧化物和次氯酸的能力方面均等同。
Several different methods are currently used by numerous laboratories for the isolation of human neutrophils. These methods include partial purification by dextran sedimenation followed by water lysis, and more complete purification procedures utilizing discontinuous density gradients coupled with dextran sedimentation and in some cases hypotonic lysis. Some investigators refrain from using certain purification schemes because certain steps or reagents used in a particular method might adversely affect the functional parameter of the neutrophil they wish to measure. In spite of these concerns there has been no systematic comparison of the functional status of neutrophils prepared by the various methodologies. In this study we have compared 4 commonly used methods of isolation with neutrophil function. The results of this study indicate that while neutrophil yield and purity were determined by isolation procedure, all cells were equivalent with regard to chemotactic performance, ability to degranulate, and ability to produce superoxide and hypochlorous acid.