Effects of luteinizing hormone and follicle-stimulating hormone and insulin-like growth factor-I on aromatase activity and P450 aromatase gene expression in the ovarian follicles of red seabream, Pagrus major
Effects of luteinizing hormone and follicle-stimulating hormone and insulin-like growth factor-I on aromatase activity and P450 aromatase gene expression in the ovarian follicles of red seabream, Pagrus major
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DOI：
10.1095/biolreprod.102.008219
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发表时间：
2003-05-01
影响因子：
3.6
通讯作者：
Tanaka, H
中科院分区：
文献类型：
--
作者：
Kagawa, H;Gen, K;Tanaka, H
为阐明红鲷卵巢卵泡产生雌二醇-17 β的机制，在体外研究了促黄体生成素（LH）、促卵泡生成素（FSH）和胰岛素样生长因子（IGF-1）对红鲷卵巢片段芳香化酶活性（睾酮转化为雌二醇-17 β）和细胞色素P450芳香化酶（P450arom） mRNA表达的影响。在本研究中使用的生长因子中，只有IGF-1能同时刺激红鲷卵巢片段的芳香酶活性和P450arom基因表达。红鲷垂体LH能刺激芳香酶活性和P450arom基因表达，而FSH不能。IGF-1轻微增强了lh诱导的芳香酶活性和P450arom基因表达。这些数据和我们之前的结果表明，LH而不是FSH通过刺激芳香酶活性和P450arom基因表达来刺激红鲷卵巢卵泡中雌二醇-17 β的产生，而IGF-1增强了LH刺激的P450arom基因表达。
To clarify the mechanism of estradiol-17beta production in the ovarian follicle of red seabream, in vitro effects of luteinizing hormone (LH), follicle-stimulating hormone (FSH), and insulin-like growth factor (IGF-1) on aromatase activity (conversion of testosterone to estradiol-17beta) and cytochrome P450 aromatase (P450arom) mRNA expression in ovarian fragments of red seabream were investigated. Of the growth factors used in the present study, only IGF-1 stimulated both aromatase activity and P450arom gene expression in the ovarian fragments of red seabream. LH from red seabream pituitary, but not FSH, stimulated both aromatase activity and P450arom gene expression. IGF-1 slightly enhanced the LH-induced aromatase activity and P450arom gene expression. These data and our previous results indicate that LH, but not FSH, stimulates estradiol-17beta production in the ovarian follicle of red seabream through stimulation of aromatase activity and P450arom gene expression and IGF-1 enhances the LH-stimulated P450arom gene expression.