THE ACTION OF NITRIC-OXIDE IN THE PERFUSED HUMAN FETAL-PLACENTAL CIRCULATION
THE ACTION OF NITRIC-OXIDE IN THE PERFUSED HUMAN FETAL-PLACENTAL CIRCULATION
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DOI：
10.1016/s0002-9378(11)80047-5
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发表时间：
1991-02-01
影响因子：
9.8
通讯作者：
BROCKMAN, DE
中科院分区：
文献类型：
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作者：
MYATT, L;BREWER, A;BROCKMAN, DE
一氧化氮被认为是内源性内皮衍生的松弛因子。 我们研究了在细胞内产生一氧化氮或抑制其对人胎盘绒毛血管系统作用的化合物的影响。 添加亚甲蓝（10（-5）mol/L）灌注介质（一种一氧化氮激活鸟苷酸环化酶的抑制剂），与没有亚甲蓝的情况下观察到的灌注压力相比，在一定流速（1至10ml/min）范围内胎儿胎盘循环的灌注压力显着增加。 这表明一氧化氮的基础释放可能有助于维持静息血管张力。 三硝酸甘油酯（10(-9) 至 5 x 10(-6) mol/L）和 S-亚硝基-N-乙酰青霉胺（10(-8) 至 10(-4) mol/L）在细胞内产生一氧化氮，能够显着舒张血栓素模拟物 U46619 预收缩的胎儿胎盘循环（1 至 5 x 10(-8) mol/L)，呈浓度依赖性。 这些化合物在没有血管收缩剂的情况下没有作用。 因此，胎盘绒毛血管树似乎具有产生一氧化氮并对其做出反应的能力。
Nitric oxide is thought to be the endogenous endothelium-derived relaxing factor. We investigated the effects of compounds that either generate nitric oxide intracellularly or inhibit its action on the vasculature of the human placental villus. Addition to perfusion medium of methylene blue (10(-5) mol/L), which is an inhibitor of activation of guanylate cyclase by nitric oxide, significantly increased perfusion pressure of the fetal-placental circulation over a range of flow rates (1 to 10 ml/min) compared with the perfusion pressures seen in the absence of methylene blue. This suggests basal release of nitric oxide may contribute to maintenance of resting vascular tone. Both glyceryl trinitrate (10(-9) to 5 x 10(-6) mol/L) and S-nitroso-N-acetylpenicillamine (10(-8) to 10(-4) mol/L), which generate nitric oxide intracellularly, were able to significantly vasodilate the fetal-placental circulation preconstricted with the thromboxane mimetic U46619 (1 to 5 x 10(-8) mol/L) in a concentration-dependent manner. These compounds had no effect in the absence of the vasoconstrictor. Thus it appears that the placental villus vascular tree has the ability to both generate and respond to nitric oxide.