Raster-Mode Continuous-Flow Liquid Microjunction Mass Spectrometry Imaging of Proteins in Thin Tissue Sections
Raster-Mode Continuous-Flow Liquid Microjunction Mass Spectrometry Imaging of Proteins in Thin Tissue Sections
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DOI：
10.1021/acs.analchem.7b00977
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发表时间：
2017-06-06
影响因子：
7.4
通讯作者：
Cooper, Helen J.
中科院分区：
文献类型：
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作者：
Griffiths, Rian L.;Randall, Elizabeth C.;Cooper, Helen J.
先前已经演示了在离散位置（阵列模式）使用连续流液体微连接采样的质谱成像。在这封信中，我们展示了以光栅模式对薄组织切片中的蛋白质进行连续流液体微连接质谱成像，并讨论了其优点（分析时间缩短了 10 倍）。该方法的挑战（合适的溶剂系统、数据解释）：：数据可视化并非易事，需要溶剂流量、质谱数据采集速率、数据质量和液体微连接采样面积的相关性。后者对于确定最佳像素尺寸尤其重要。可实现的最小像素大小与所用仪器的扫描时间有关。此处，我们展示了使用 Orbitrap Elite 时可实现的最小像素尺寸为 50 μ m（x 维度）；然而，建议使用 600 μm 的像素大小，以尽量减少过采样对图像精度的影响。
Mass spectrometry imaging by use of continuous-flow liquid micro junction sampling at discrete locations (array mode) has previously been demonstrated. In this Letter, we demonstrate continuous-flow liquid microjunction mass spectrometry imaging of proteins from thin tissue sections in raster mode and discuss advantages (a 10-fold seduction in analysis time) and. challenges (suitable solvent systems, data interpretation) of the approach:: Visualization of data is nontrivial, requiring correlation of solvent-flow, mass spectral data acquisition rate, data quality, and liquid microjunction sampling area. The latter is particularly important for determining optimum pixel size. The minimum achievable pixel size is related to the scan time of the instrument used. Here we show a minimum achievable pixel size of 50 mu m (x-dimension) when using an Orbitrap Elite; however a pixel size of 600 mu m is recommended in order to minimize the effects of oversampling oh image accuracy.