New molecular packing in a crystal of pseudoazurin from Alcaligenes faecalis: a double-helical arrangement of blue copper.
New molecular packing in a crystal of pseudoazurin from Alcaligenes faecalis: a double-helical arrangement of blue copper.
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来自粪产碱菌的伪天青晶体中的新分子堆积：蓝铜的双螺旋排列。
DOI：
10.1107/s2053230x17002631
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Eiichi Mizohata and Tsuyoshi Inoue
中科院分区：
文献类型：
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作者：
Yohta Fukuda;Eiichi Mizohata and Tsuyoshi Inoue
假天青蛋白是一种蓝铜蛋白，是含铜亚硝酸盐还原酶的电子供体。常规地，AfPAz已经使用高度浓缩的硫酸铵作为沉淀剂结晶。在这里，针状晶体AfPAz生长在含有大分子沉淀剂，聚乙二醇8000（PEG 8000）的溶液中，报告。该晶体属于空间群P61，晶胞参数a = B = 68.7，c = 94.2 Å。 结构已确定，并在2.6 μ m的分辨率进行了改进。 不对称单元含有两个AfPAz分子，它们在带负电荷的表面上彼此接触。晶体的分子包装显示了右手双螺旋排列的AfPAz分子，因此蓝色铜网站。该结构提供了对PEG的排斥体积效应和AfPAz的组装方式的洞察。
Pseudoazurin from the denitrifying bacterium Alcaligenes faecalis (AfPAz) is a blue copper protein and functions as an electron donor to copper-containing nitrite reductase (CuNIR). Conventionally, AfPAz has been crystallized using highly concentrated ammonium sulfate as a precipitant. Here, a needle-like crystal of AfPAz grown in a solution containing a macromolecular precipitant, polyethylene glycol 8000 (PEG 8000), is reported. The crystal belonged to space group P61, with unit-cell parameters a = b = 68.7, c = 94.2 Å. The structure has been determined and refined at 2.6 Å resolution. The asymmetric unit contained two AfPAz molecules contacting each other on negatively charged surfaces. The molecular packing of the crystal showed a right-handed double-helical arrangement of AfPAz molecules and hence of blue copper sites. This structure provides insight into the excluded-volume effect of PEG and the manner of assembly of AfPAz.