NDM-1 encoded by a pNDM-BJ01-like plasmid p3SP-NDM in clinical Enterobacter aerogenes.
NDM-1 encoded by a pNDM-BJ01-like plasmid p3SP-NDM in clinical Enterobacter aerogenes.
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DOI：
10.3389/fmicb.2015.00294
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发表时间：
2015
影响因子：
5.2
通讯作者：
Yin Z
中科院分区：
文献类型：
--
作者：
Chen Z;Li H;Feng J;Li Y;Chen X;Guo X;Chen W;Wang L;Lin L;Yang H;Yang W;Wang J;Zhou D;Liu C;Yin Z
从某教学医院1例肺炎患者中分离到一株对碳青霉烯类抗生素不敏感的产气肠杆菌3-SP。NDM-1碳青霉烯酶由pNDM-BJ 01样接合质粒p3 SP-NDM产生，以解释3-SP的碳青霉烯耐药性。p3 SP-NDM和pNDM-BJ 01之间的遗传差异仅包括18个单核苷酸多态性，1个bp缺失和706个bp缺失。p3 SP-NDM和pNDM-BJ 01含有相同的Tn 125元件，组织为ISAba 125、blaNDM-1、bleMBL、ΔtrpF、dsbC、cutA、ΔgroES、groEL、ISCR 27和ISAba 125。这些pNDM-BJ 01样质粒中blaNDM−1周围区域具有保守的线性组织ISAba 14-aphA 6-Tn 125-unknown IS，在Tn 125内或紧邻Tn 125下游鉴定出相当大的遗传差异。所有已报道的pNDM-BJ 01样质粒仅在不动杆菌中发现，而这是首次在肠杆菌科中鉴定出pNDM-BJ 01样质粒。
A carbapenem-nonsusceptible Enterobacter aerogenes strain named 3-SP was isolated from a human case of pneumonia in a Chinese teaching hospital. NDM-1 carbapenemase is produced by a pNDM-BJ01-like conjugative plasmid designated p3SP-NDM to account for carbapenem resistance of 3-SP. p3SP-NDM was fully sequenced and compared with all publically available pNDM-BJ01-like plasmids. The genetic differences between p3SP-NDM and pNDM-BJ01 include only 18 single nucleotide polymorphisms, a 1 bp deletion and a 706 bp deletion. p3SP-NDM and pNDM-BJ01 harbor an identical Tn125 element organized as ISAba125, blaNDM−1, bleMBL, ΔtrpF, dsbC, cutA, ΔgroES, groEL, ISCR27, and ISAba125. The blaNDM−1 surrounding regions in these pNDM-BJ01-like plasmids have a conserved linear organization ISAba14-aphA6-Tn125-unknown IS, with considerable genetic differences identified within or immediately downstream of Tn125. All reported pNDM-BJ01-like plasmids are exclusively found in Acinetobacter, whereas this is the first report of identification of a pNDM-BJ01-like plasmid in Enterobacteriaceae.