Horizontal transfer of DNA by the uptake of apoptotic bodies
Horizontal transfer of DNA by the uptake of apoptotic bodies
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DOI：
10.1182/blood.v93.11.3956.411k05_3956_3963
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发表时间：
1999-06-01
期刊：
影响因子：
20.3
通讯作者：
Falk, KI
中科院分区：
文献类型：
--
作者：
Holmgren, L;Szeles, A;Falk, KI
在这项研究中，我们提出了DNA是否可以通过吞噬凋亡小体从一个细胞转移到另一个细胞的问题。我们使用EB病毒(EBV)的整合拷贝作为标记，以跟踪吞噬宿主中凋亡DNA的命运和表达模式。携带EB病毒的细胞系在与人成纤维细胞、巨噬细胞或牛主动脉内皮细胞培养前通过照射诱导细胞凋亡。免疫荧光染色和原位杂交分析EBV编码基因的表达模式。从含有EBV整合体而不是异体拷贝的淋巴细胞系中共培养出的凋亡体导致EBV编码基因EBER和EBNA1在受体细胞中的高频率表达。荧光原位杂交分析表明，牛主动脉内皮细胞摄取了人染色质并整合了EBV-DNA，这些数据表明DNA可能被体细胞从凋亡的小体中回收和重新利用。此外，我们的发现表明，来自携带EBV的B淋巴细胞的凋亡体可能是病毒转移到体内缺乏EBV病毒受体的细胞的来源。(C)1999，美国血液学学会。
In this study we have raised the question of whether DNA can be transferred from one cell to another by phagocytosis of apoptotic bodies. We have used integrated copies of the Epstein-Barr virus (EBV) as a marker to follow the fate and expression pattern of apoptotic DNA in the phagocytotic host. Apoptosis was induced in EBV-carrying cell lines by irradiation before cultivation with either human fibroblasts, macrophages, or bovine aortic endothelial cells. Analysis of the expression pattern of EBV-encoded genes was performed by immunofluorescent staining as well as in situ hybridization. Cocultivation of apoptotic bodies from lymphoid cell lines containing integrated but not episomal copies of EBV resulted in expression of the EBV-encoded genes EBER and EBNA1 in the recipient cells at a high frequency. Fluorescence in situ hybridization analysis showed uptake of human chromatin as well as integrated EBV-DNA into the nuclei of bovine aortic endothelial cells, These data show that DNA may be rescued and reused from apoptotic bodies by somatic cells. In addition, our findings suggest that apoptotic bodies derived from EBV-carrying B lymphocytes may serve as the source of viral transfer to cells that lack receptors for the EBV virus in vivo, (C) 1999 by The American Society of Hematology.