Multi and mixed 3D-printing of graphene-hydroxyapatite hybrid materials for complex tissue engineering
Multi and mixed 3D-printing of graphene-hydroxyapatite hybrid materials for complex tissue engineering
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DOI：
10.1002/jbm.a.35684
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发表时间：
2017-01-01
影响因子：
4.9
通讯作者：
Shah, Ramille. N.
中科院分区：
文献类型：
--
作者：
Jakus, Adam E.;Shah, Ramille. N.
随着三维(3D)打印(3DP)作为组织工程和医学中的重要工具的出现，人们越来越需要开发能够进行3D打印并模拟人体组织和器官的成分、结构和功能的新的生物材料。在这项工作中，我们通过结合最近建立的两种功能3D可打印颗粒生物材料墨水来探索3D可打印生物材料光谱：一种包含羟基磷灰石微球(超弹性骨，HB)，另一种包含石墨烯纳米片(3D-石墨烯，3DG)。我们证明，这些独特的、成骨的和神经生成的墨水不仅可以通过3D打印来创建复杂的多材料结构，而且还可以合成HB和3DG的复合墨水。具体地，研究了体积比为1：1的HA：石墨烯杂化材料的印刷适性、微观结构、机械、电和生物性能。由此产生的HB-3DG混合系统展示了两个不同系统的混合特征，同时保持了3D可打印性、导电性和灵活性。使用间充质干细胞对HB-3DG进行的体外评估表明，这种杂化材料支持细胞的存活和增殖，并在14天内显著上调成骨和神经源基因的表达。这项工作最终展示了朝着能够从用于复杂复合组织工程的混合墨水中3D打印分级、多组分和多功能构造的方向迈出了重要的一步。(C)2016 Wiley期刊，Inc.生物材料资源A部分：105A：274-283,2017。
With the emergence of three-dimensional (3D)-printing (3DP) as a vital tool in tissue engineering and medicine, there is an ever growing need to develop new biomaterials that can be 3D-printed and also emulate the compositional, structural, and functional complexities of human tissues and organs. In this work, we probe the 3D-printable biomaterials spectrum by combining two recently established functional 3D-printable particle-laden biomaterial inks: one that contains hydroxyapatite microspheres (hyperelastic bone, HB) and another that contains graphene nanoflakes (3D-graphene, 3DG). We demonstrate that not only can these distinct, osteogenic, and neurogenic inks be co-3D-printed to create complex, multimaterial constructs, but that composite inks of HB and 3DG can also be synthesized. Specifically, the printability, microstructural, mechanical, electrical, and biological properties of a hybrid material comprised of 1:1 HA:graphene by volume is investigated. The resulting HB-3DG hybrid exhibits mixed characteristics of the two distinct systems, while maintaining 3D-printability, electrical conductivity, and flexibility. In vitro assessment of HB-3DG using mesenchymal stem cells demonstrates the hybrid material supports cell viability and proliferation, as well as significantly upregulates both osteogenic and neurogenic gene expression over 14 days. This work ultimately demonstrates a significant step forward towards being able to 3D-print graded, multicompositional, and multifunctional constructs from hybrid inks for complex composite tissue engineering. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 274-283, 2017.