Long persistence of monofunctional 8-methoxypsoralen-DNA adducts in human skin in vivo.
Long persistence of monofunctional 8-methoxypsoralen-DNA adducts in human skin in vivo.
复制标题
单功能 8-甲氧基补骨脂素-DNA 加合物在体内人体皮肤中的长期持久性。
DOI：
10.1111/j.1751-1097.1991.tb02069.x
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发表时间：
1991
影响因子：
3.3
通讯作者：
Gange,RW
中科院分区：
文献类型：
--
作者：
Ortel,B;Maytum,DJ;Gange,RW
人体皮肤可以通过局部应用8-甲氧基补骨脂素水溶液并立即进行非红斑剂量的380 nm以上的照射来持续光敏。如此敏化的皮肤在72-120小时后再次暴露于宽带UV-A会产生光毒性红斑。再次曝光后的光毒性红斑证明了光敏作用的持久性，直到第一次敏化照射后15天。根据第一次暴露主要诱导补骨脂素单加合物的概念，我们认为这是对补骨脂素单加合物持久性的研究。与早期的几项研究不同，这种灵敏的方法表明，补骨脂素单加合物在形成后可能会在人皮肤活体中保留2周以上。
Human skin can be persistently photosensitized by topical application of aqueous 8‐methoxypsoralen plus immediate irradiation with a non‐erythemogenic dose of wavelengths above 380 nm. Re‐exposure of skin thus sensitized to broadband UV‐A produces phototoxic erythema72–120 h later. The persistence of the photosensitization was demonstrated by phototoxic erythema after re‐exposure up to 15 days after the first sensitizing irradiation. According to the concept that the first exposure induces primarily psoralen monoadducts, we consider this an investigation of psoralen monoadduct persistence. In contrast to several earlier studies, this sensitive method indicates that psoralen monoadducts may remain in human skinin vivofor more than 2 weeks after formation.