Inhibition of Hyaluronic Acid Synthesis Decreases Endometrial Cell Attachment, Migration, and Invasion.
Inhibition of Hyaluronic Acid Synthesis Decreases Endometrial Cell Attachment, Migration, and Invasion.
复制标题
抑制透明质酸合成可减少子宫内膜细胞的附着、迁移和侵袭。
DOI：
10.1007/s43032-019-00100-w
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Knudtson,JenniferF
中科院分区：
文献类型：
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作者：
McLaughlin,JessicaE;Santos,MarlenTellez;Binkley,PeterA;Sultana,Mubeen;Tekmal,RajeshwarR;Schenken,RobertS;Knudtson,JenniferF
为了表征4-甲基伞形酮（4-MU）对透明质酸（HA）系统的表达以及对子宫内膜上皮（EECs）和基质细胞（ESCs）向腹膜间皮细胞（PMCs）的附着、迁移和侵袭的影响，在学术中心进行了这项体外研究。使用的去识别子宫内膜组织样本来自育龄妇女。从月经子宫内膜活检中分离的EEC和ESC用4-MU或载体处理。实时聚合酶链反应和蛋白质印迹法用于评估HA酶（HAS）、透明质酸酶和标准CD 44的表达。使用已建立的体外测定来评估用和不用4-MU处理的附着、迁移和侵袭。适当时，使用卡方和Student t检验分析结果。与对照组相比，4-MU的加入降低了EECs和ESC中HAS 2、HAS 3和CD 44的mRNA和蛋白表达。与对照组相比，4-MU治疗也减少了EECs和ESCs对PMC的附着、迁移和侵袭。4-MU降低子宫内膜细胞对PMC的粘附、迁移和侵袭。这种作用似乎是由HAS 2、HAS 3和CD 44的减少介导的。4-MU是子宫内膜异位症的潜在治疗方法。未来的体内研究需要评估4-MU作为子宫内膜异位症的治疗药物。
To characterize the effects of 4-methylumbelliferone (4-MU) on expression of the hyaluronic acid (HA) system and on attachment, migration, and invasion of endometrial epithelial (EECs) and stroma cells (ESCs) to peritoneal mesothelial cells (PMCs), this in vitro study was performed in an Academic Center. De-identified endometrial tissue samples used were from reproductive-aged women. EECs and ESCs isolated from menstrual endometrial biopsies were treated with 4-MU or vehicle. Real-time polymerase chain reaction and western blot were used to assess expression of HA synthases (HAS), hyaluronidase, and standard CD44. Established in vitro assays were used to assess attachment, migration, and invasion with and without treatment with 4-MU. Chi square and Student’s t-test were used to analyze the results as appropriate. The addition of 4-MU decreased mRNA and protein expression of HAS 2, HAS 3, and CD44 in EECs and ESCs compared to control. Treatment with 4-MU also decreased attachment, migration, and invasion of EECs and ESCs to PMCs compared to control. 4-MU decreases endometrial cell adhesion, migration, and invasion to PMCs. This effect appears to be mediated by a decrease in HAS 2, HAS 3, and CD44. 4-MU is a potential treatment for endometriosis. Future in vivo studies are needed to evaluate 4-MU as a therapeutic agent for endometriosis.