Isolation and characterization of three maize aquaporin genes, ZmNIP2;1, ZmNIP2;4 and ZmTIP4;4 involved in urea transport
Isolation and characterization of three maize aquaporin genes, ZmNIP2;1, ZmNIP2;4 and ZmTIP4;4 involved in urea transport
复制标题
参与尿素转运的三个玉米水通道蛋白基因 ZmNIP2;1、ZmNIP2;4 和 ZmTIP4;4 的分离和表征
DOI：
10.5483/bmbrep.2012.45.2.96
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发表时间：
2012-02-29
期刊：
影响因子：
3.8
通讯作者：
Yuan, Lixing
中科院分区：
文献类型：
--
作者：
Gu, Riliang;Chen, Xiaoling;Yuan, Lixing
尿素氮肥在玉米生产中得到了广泛应用，但与尿素吸收、转运和细胞内稳态有关的转运蛋白尚未被鉴定。本研究利用尿素吸收缺陷型酵母ZmNI 132; 1和ZmNIP 2;4的异源互补，从cDNA文库中分离到3个玉米aquapoin基因，ZmNIP 2;1、ZmNIP 2;4和ZmTIP 4;4，它们属于位于质膜上的类胰蛋白酶26内在蛋白（NIPs），而ZmT 1 P4;4属于液泡膜上的液泡膜内在蛋白（TIPs）。定量RT-PCR结果表明，ZmNIP 2;1在各器官中组成型表达，而ZmNIP 2;4和ImT 1 P4;4在生殖器官和根中表达丰富。ZmTIP 4的表达; ZmNIP 2; 1和ZmNIP 2; 4在氮饥饿条件下的根和展开叶的生长量显著增加，而ZmNIP 2;1和ZmNIP 2;4的根和展开叶的生长量没有受到影响。玉米aquapoin基因在尿素转运中的功能及其不同的表达方式表明，它们可能在尿素的吸收和转运，或平衡液泡膜中的尿素浓度方面发挥不同的作用。[BMB报告2012; 45（2）：96-101]
Urea-based nitrogen fertilizer was widely utilized in maize production, but transporters involved in urea uptake, translocation and cellular homeostasis have not been identified. Here, we isolated three maize aquapoin genes, ZmNIP2;1, ZmNIP2;4 and ZmTIP4;4, from a cDNA library by heterogous complementation of a urea uptake-defective yeast ZmNI132;1 and ZmNIP2;4 belonged to the nodulin 26-like intrinsic proteins (NIPs) localized at plasma membrane, and ZmT1P4;4 belonged to the tonoplast intrinsic protein (TIPs) at vacuolar membrane. Quantitative RT-PCR revealed that ZmNIP2;1 was expressed constitutively in various organs while ZmNIP2;4 and ImT1P4;4 transcripts were abundant in reproductive organs and roots. Expression of ZmTIP4;4 was significantly increased in roots and expanded leaves under nitrogen starvation, while those of ZmNIP2;1 and ZmNIP2;4 remained unaffected. Functions of maize aquapoin genes in urea transport together with their distinct expression manners suggested that they might play diverse roles on urea uptake and translocation, or equilibrating urea concentration across tonoplast. [BMB reports 2012; 45(2): 96-101]