Effects of hydroquinone and its glucoside derivatives on melanogenesis and antioxidation: Biosafety as skin whitening agents
Effects of hydroquinone and its glucoside derivatives on melanogenesis and antioxidation: Biosafety as skin whitening agents
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DOI：
10.1016/j.jdermsci.2009.06.003
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发表时间：
2009-09-01
影响因子：
4.6
通讯作者：
Xu, Shi-Zheng
中科院分区：
文献类型：
--
作者：
Hu, Zhi-Ming;Zhou, Qiong;Xu, Shi-Zheng
背景与目的：对苯二酚及其衍生物作为皮肤增白剂的生物安全性仍存在争议。在这里，我们研究了对苯二酚、熊果苷和脱氧熊果苷（D-arb）对黑色素形成和抗氧化的影响，使用培养的黑色素细胞，在存在或不存在紫外线A （UVA）诱导的氧化应激的情况下，并确定D-arb是否能够替代对苯二酚和熊果苷用于皮肤美白。方法：本研究通过去除熊果苷葡萄糖侧链上的所有羟基合成D-arb。用c -14-酪氨酸结合法测定酪氨酸酶活性，用H(2)DCFDA荧光标记法监测细胞内活性氧（ROS）水平，用MTT法测定在uva诱导的氧化应激存在或不存在的情况下，对苯二酚、熊果苷和脱氧熊果苷处理的小鼠黑色素细胞的细胞活力。结果：在无毒性剂量（3 J/cm(2)）的UVA照射下，除D-arb外，对苯二酚和熊果苷的细胞毒性均增强。与熊果苷和对苯二酚相比，D-arb处理可抑制ROS生成。所有三种化合物都具有相似的抑制油酪氨酸酶活性的剂量依赖方式，与未处理的对照相比降低了2至3倍。熊果苷和对苯二酚处理的细胞中酪氨酸酶蛋白表达没有变化，但脱氧熊果苷处理的细胞中酪氨酸酶蛋白表达下降。结论：脱氧熊果苷对酪氨酸酶有较强的抑制作用，具有较低的细胞毒性和一定的抗氧化潜力，可作为对苯二酚的安全有效的皮肤美白替代品。(C) 2009年日本皮肤病研究学会。爱思唯尔爱尔兰有限公司出版。版权所有。
Background and objective: The biosafety of hydroquinone and its derivatives as skin whitening agent remains controversial. Here, we investigated the effects of hydroquinone, arbutin, and deoxyarbutin (D-arb) on melanogenesis and antioxidation Using Cultured melan-a melanocytes in the presence or absence of ultraviolet A (UVA)-induced oxidative stress and determined whether D-arb enables to be an alterative to hydroquinone and arbutin for skin whitening use.Methods: D-arb was synthesized ill this Study by removing all hydroxyl groups from the glucose side-chain of arbutin. Tyrosinase activity was measured by C-14-tyrosine incorporation, the intracellular reactive oxygen species (ROS) level was monitored by H(2)DCFDA fluorescence labeling, and [lie cell viability was determined by MTT assay in murine melan-a melanocytes treated with hydroquinone, arbutin and deoxyarbutin in the presence or absence of UVA-induced oxidative stress.Results: The cytotoxicity of hydroquinone and arbutin except for D-arb was increased while the cells exposed to a nontoxic dose (3 J/cm(2)) of UVA irradiation. Suppressed ROS generation was noted by the treatment Of D-arb to compare with arbutin and hydroquinone. All three compounds had a similar inhibition oil tyrosinase activity in dose-dependent manners with two- to three-fold decreases over the untreated control. There was no change in expression of tyrosinase protein in cells treated with arbutin or hydroquinone, but a decreased protein expression of tyrosinase was seen in deoxyarbutin-treated cells.Conclusions: Deoxyarbutin exerts potent tyrosinase inhibition, lessened cytotoxicity, and certain antioxidation potential, may serve as an effective and safe alternative to hydroquinone for use in skin whitening. (C) 2009 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.