Molecular cloning and mapping of the human nicotinic acetylcholine receptor α10 (CHRNA10)
Molecular cloning and mapping of the human nicotinic acetylcholine receptor α10 (CHRNA10)
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DOI：
10.1006/geno.2000.6503
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发表时间：
2001-05-01
期刊：
影响因子：
4.4
通讯作者：
Fuchs, PA
中科院分区：
文献类型：
--
作者：
Lustig, LR;Peng, H;Fuchs, PA
我们报告的分离和初步鉴定的人烟碱乙酰胆碱受体（nAChR）亚基家族，α 10（CHRNA 10），从内耳神经上皮和淋巴组织的新成员。cDNA长度为1959个核苷酸，编码区预测451个氨基酸的蛋白质与大鼠α 10具有90%的同一性。α 10基因定位于染色体11p15.5。应用逆转录-聚合酶链反应、北方印迹杂交和免疫组织化学方法检测人内耳组织、扁桃体、永生化B细胞、培养的T细胞和外周血淋巴细胞中的人α 10。我们还用RT-PCR和北方印迹杂交检测了人nAChR α 9（CHRNA 9）mRNA在这些相同组织中的表达。(C)北京：科学出版社.
We report the isolation and initial characterization of a new member of the human nicotinic acetylcholine receptor (nAChR) subunit family, alpha 10 (CHRNA10), from both inner-ear neuroepithelium and lymphoid tissue. The cDNA is 1959 nucleotides in length, with a coding region predicting a protein of 451 amino acids that is 90% identical to rat alpha 10. The alpha 10 gene was localized to chromosome 11p15.5. Human alpha 10 was detected inhuman inner-ear tissue, tonsil, immortalized B-cells, cultured T-cells and peripheral blood lymphocytes using reverse transcriptase-polymerase chain reaction, Northern blot hybridization, and immunohistochemistry. We also detected the expression of the human nAChR alpha9 (CHRNA9) mRNA in these same tissues using RT-PCR and Northern blot hybridization. (C) 2001 Academic Press.