piezo2b Regulates Vertebrate Light Touch Response
piezo2b Regulates Vertebrate Light Touch Response
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DOI：
10.1523/jneurosci.0522-13.2013
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发表时间：
2013-10-23
影响因子：
5.3
通讯作者：
Jopling, Chris
中科院分区：
文献类型：
--
作者：
Faucherre, Adele;Nargeot, Joel;Jopling, Chris
触觉使生物体能够探测到物理环境的刺激并作出反应。机械敏感蛋白在这一过程中起着至关重要的作用，将机械提示转化为生物反应。最近，拉伸激活离子通道的压电家族已被确定为真正的机械敏感蛋白。我们开始确定在斑马鱼发育过程中是否有这些基因参与了触摸反应。原位杂交表明，piezo2b在负责检测光触摸的神经元（罗洪-比尔德细胞）的一个子集中特异性表达。使用morpholino介导的敲除，我们专门针对piezo2b，并确定它参与介导触摸诱发反应。
The sense of touch allows an organism to detect and respond to physical environmental stimuli. Mechanosensitive proteins play a crucial role in this process by converting the mechanical cue into a biological response. Recently, the Piezo family of stretch-activated ion channels has been identified as genuine mechanosensitive proteins. We set out to determine whether any of these genes are involved in touch response during zebrafish development. In situ hybridization indicates that piezo2b is specifically expressed in a subset of neurons (Rohon-Beard cells) responsible for detecting light touch. Using morpholino-mediated knockdown, we specifically targeted piezo2b and determined that it is involved in mediating touch-evoked response.