Reversible Accumulation of PEGylated Single-Walled Carbon Nanotubes in the Mammalian Nucleus
Reversible Accumulation of PEGylated Single-Walled Carbon Nanotubes in the Mammalian Nucleus
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DOI：
10.1021/nn800461u
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发表时间：
2008-10-01
期刊：
影响因子：
17.1
通讯作者：
Cheng, Shuk Han
中科院分区：
文献类型：
--
作者：
Cheng, Jinping;Fernando, K. A. Shiral;Cheng, Shuk Han
碳纳米管(CNTs)可以穿透细胞膜，并能介导大分子的内化。这些特性构成了碳纳米管作为一种令人兴奋的药物输送和生物传感的新工具。虽然碳纳米管在生物医学和医药领域显示出巨大的应用潜力，但碳纳米管的细胞穿透机制和内化的碳纳米管在细胞内的去向还不完全清楚。在这项研究中，利用时间推移荧光显微镜研究了FITC标记的聚乙二醇化单壁碳纳米管在活细胞内的分布，结果表明，聚乙二醇化的单壁碳纳米管以能量依赖的方式进入几种哺乳动物细胞系的细胞核。FITC-聚乙二醇单壁碳纳米管在细胞核中的存在不会引起核组织的明显变化，并且在长达5天的时间内对细胞的生长动力学和细胞周期分布没有影响。值得注意的是，当FITC-PEG-SWCNTs从培养液中移除后，内化的FITC-PEG-SWCNTs迅速移出细胞核，并从细胞中释放出来。因此，细胞内的聚乙二醇化的单壁碳纳米管是高度动态的，并且聚乙二醇化的单壁碳纳米管的细胞穿透表现为双向的。这些观察结果表明，单壁碳纳米管可以作为一种理想的纳米载体应用于生物医学和医药领域。
Carbon nanotubes (CNTs) have been shown to cross cell membranes and can mediate the internalization of macromolecules. These characteristics have constituted CNTs as an exciting new tool for drug delivery and biological sensing. While CNTs exhibit great potential in biomedical and pharmaceutical applications, neither the cell penetration mechanism of CNTs nor the intracellular fate of the internalized CNTs are fully understood. In this study, time-lapse fluorescence microscopy was used to investigate the intracellular distribution of FITC labeled PEGylated single-walled CNTs (FITC-PEG-SWCNTs) in living cells and shown that PEGylated SWCNTs entered the nucleus of several mammalian cell lines in an energy-dependent process. The presence of FITC-PEG-SWCNTs in the cell nucleus did not cause discernible changes in the nuclear organization and had no effect on the growth kinetics and cell cycle distribution for up to 5 days. Remarkably, upon removal of the FITC-PEG-SWCNTS from the culture medium, the internalized FITC-PEG-SWCNTs rapidly moved out of the nucleus and were released from the cells. Thus, the intracellular PEGylated SWCNTs were highly dynamic and the cell penetration of PEGylated SWCNTs appeared as bidirectional. These observations suggest SWCNTs may be used as an ideal nanovector in biomedical and pharmaceutical applications.