Delayed treatment with nicotinamide (vitamin B3) improves neurological outcome and reduces infarct volume after transient focal cerebral ischemia in Wistar rats
Delayed treatment with nicotinamide (vitamin B3) improves neurological outcome and reduces infarct volume after transient focal cerebral ischemia in Wistar rats
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DOI：
10.1161/01.str.31.7.1679
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发表时间：
2000-07-01
期刊：
影响因子：
8.3
通讯作者：
Maynard, KI
中科院分区：
文献类型：
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作者：
Mokudai, T;Ayoub, IA;Maynard, KI
背景和目的-我们先前已经证明烟酰胺(NAM)能显著减少永久性大脑中动脉闭塞(MCAO)所致的大鼠脑梗塞。在本研究中，我们在短暂性局灶性脑缺血模型上观察了NAM是否通过改善感觉和运动行为以及脑梗塞体积对脑缺血/再灌注损伤有保护作用。方法48只雄性Wistar大鼠，用大脑中动脉结扎诱导短暂性局灶性脑缺血2小时，然后再灌流3或7天。动物在MCAO后2小时(即再灌流时)给予生理盐水或NAM(500 mg/kg)处理。结果与生理盐水治疗相比，短暂性局灶性脑缺血后2小时给予NAM(500 mg/kg IF)治疗后，Wistar大鼠感觉功能明显改善(38%，P
Background and Purpose-We have previously shown that nicotinamide (NAm) acutely reduces brain infarction induced by permanent middle cerebral artery occlusion (MCAo) in rats. In this study, we investigate whether NAm may protect against ischemia/reperfusion injury by improving sensory and motor behavior as well as brain infarction volumes in a model of transient focal cerebral ischemia.Methods-Forty-eight male Wistar rats were used, and transient focal cerebral ischemia was induced by MCAo for 2 hours, followed by reperfusion for either 3 or 7 days. Animals were treated with either intraperitoneal saline or NAm (500 mg/kg) 2 hours after the onset of MCAo (ie, on reperfusion). Sensory and motor behavior scores and body weight were obtained daily, and brain infarction volumes were measured on euthanasia.Results-Relative to treatment with saline, treatment with NAm (500 mg/kg IF) 2 hours after the onset of transient focal cerebral ischemia in Wistar rats significantly improved sensory (38%, P