Eastern Blotting Analysis and Isolation of Two New Dammarane-Type Saponins from American Ginseng
Eastern Blotting Analysis and Isolation of Two New Dammarane-Type Saponins from American Ginseng
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DOI：
10.1248/cpb.c12-00486
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发表时间：
2012-10-01
影响因子：
1.7
通讯作者：
Shoyama, Yukihiro
中科院分区：
文献类型：
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作者：
Nguyen Huu Tung;Shoyama, Yukihiro
用抗人参皂苷Rb-1和Rg(1)单克隆抗体（mab）对西洋参主要活性成分人参皂苷进行东部印迹分析。利用免疫分析法对西洋参甲醇提取物进行分离和柱层析，分离到两种新的次要达玛烷型皂苷，分别命名为quinquenosides Ja(1)和Jb(2)。通过化学和光谱方法鉴定其结构为6- o -[α - l -鼠李糖吡喃基(1 ->)- β - d -葡萄糖吡喃基]-20- o -[β - d -葡萄糖吡喃基(1 -> 4)- β - d -葡萄糖吡喃基]-3 β，6 α,12 β，20 β -四羟吡喃基]-20- o -{[α - l -阿拉伯糖吡喃基(1 -> 2)- β - d -葡萄糖吡喃基](1 -> 6)- β - d -葡萄糖吡喃基]-3 β，12 β，20 β -三羟吡喃基-24-烯(2)。
Ginsenosides, the major active component of American ginseng, were analyzed using eastern blotting with anti-ginsenoside Rb-1 and Rg(1) monoclonal antibodies (MAbs). Immunoassay-guided fractionation of the methanol extract of American ginseng and column chromatography led to the isolation of two new minor dammarane-type saponins, named quinquenosides Ja (1) and Jb (2). Their structures were elucidated to be 6-O-[alpha-L-rhamnopyranosyl(1 -> 2)-beta-D-glucopyranosyl]-20-O-[beta-D-glucopyranosyl(1 -> 4)-beta-D-glucopyranosyl]-3 beta,6 alpha,12 beta,20 beta-tetrahydroxydarnmar-24-ene (1) and 3-O-[beta-D-glucopyranosyl(1 -> 2)-beta-D-glucopyranosyl]-20-O-{[alpha-L-arabinofuranosyl(1 ->>6)-beta-D-glucopyranosyl](1 -> 6)-beta-D-glucopyranosyl]-3 beta,12 beta,20 beta-trihydroxydammar-24-ene (2) on the basis of chemical and spectroscopic methods.