ELEVATED EXPRESSION OF CD80 (B7/BB1) AND OTHER ACCESSORY MOLECULES ON SYNOVIAL-FLUID MONONUCLEAR CELL SUBSETS IN RHEUMATOID-ARTHRITIS
ELEVATED EXPRESSION OF CD80 (B7/BB1) AND OTHER ACCESSORY MOLECULES ON SYNOVIAL-FLUID MONONUCLEAR CELL SUBSETS IN RHEUMATOID-ARTHRITIS
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DOI：
10.1002/art.1780371113
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发表时间：
1994-11-01
影响因子：
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通讯作者：
KIPPS, TJ
中科院分区：
文献类型：
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作者：
RANHEIM, EA;KIPPS, TJ
Objective.目的探讨类风湿关节炎（RA）患者滑液（SF）中潜在抗原呈递细胞（APC）表面重要辅助分子CD 80（B7/BB 1）和CD 54（intercellular adhesion molecule 1）的表达。通过多参数流式细胞术分析，将RA SF的T细胞、单核细胞和CD 5+或CD 5- B细胞上各种辅助分子的表达水平与来自相同患者和正常对照的外周血（PB）单核细胞上的表达进行比较。通过不同细胞群刺激混合淋巴细胞反应中T细胞的能力来评估这些辅助分子表达增加的功能意义。从RA患者炎性关节SF中分离的单核细胞、T细胞和B细胞表达的CD 80和CD 54水平显著高于从RA患者或正常对照PB中分离的那些。SF B细胞的CD 11b和CD 11 c表达也增加，而SF单核细胞的CD 40水平升高。此外，我们发现RA SF细胞中升高的CD 80和CD 54水平可能增强了这些细胞作为刺激APC的能力。这是第一次证明特定的细胞亚群在自身免疫病理学部位组成性表达增加的CD 80水平，这可能有助于引发或维持自身免疫性疾病。
Objective. To assess the expression of important accessory molecules such as CD80 (B7/BB1) and CD54 (intercellular adhesion molecule 1) on potential antigen-presenting cells (APC) in the synovial fluid (SF) of patients with rheumatoid arthritis (RA).Methods. The level of expression of various accessory molecules on T cells, monocytes, and CD5+ or CD5- B cells from RA SF was compared, by multiparameter flow cytometric analysis, with the expression on peripheral blood (PB) mononuclear cells from the same patients and from normal controls. The functional significance of increased expression of these accessory molecules was assessed by the ability of the different cell populations to stimulate T cells in the mixed lymphocyte reaction.Results. Monocytes, T cells, and B cells isolated from the SF of inflamed joints of patients with RA expressed significantly higher levels of CD80 and CD54 than those from the PB of RA patients or normal controls. CD11b and CD11c expression also were increased on SF B cells, while SF monocytes exhibited enhanced levels of CD40. Further, we found that the elevated CD80 and CD54 levels in RA SF cells may enhance the capacity of these cells to act as stimulatory APC.Conclusion. This is the first demonstration that specific cell subsets constitutively express increased levels of CD80 at a site of autoimmune pathology, which could potentially contribute to the initiation or maintenance of autoimmune disease.