Surfactant Protein A can Affect the Surface Tension of the Eustachian Tube and Macrophage Migration
Surfactant Protein A can Affect the Surface Tension of the Eustachian Tube and Macrophage Migration
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DOI：
10.1002/lary.30396
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发表时间：
2022-09-14
期刊：
影响因子：
2.6
通讯作者：
Li，Li
中科院分区：
文献类型：
--
作者：
Li，Guodong;Chen，Tao;Li，Li
目的探讨表面活性蛋白A（SPA）在分泌性中耳炎（OME）发病中的作用及其可能机制。对照组和研究组（OME组）分别通过外耳道注射磷酸盐缓冲液和灭活肺炎链球菌。测量两组咽鼓管（ET）分泌物表面张力和SPA表达的变化。采用transwell法观察不同浓度SPA对巨噬细胞迁移能力的影响。我们使用RNA-seq analysis.ResultsOn第3天，OME组的表面张力高于对照组（p= 0.014），研究了与SPA处理的巨噬细胞相关的差异表达基因。OME组ET中SPA的变化强度明显低于对照组（p< 0.001）。表面张力与SPA相关（r=-0.525，p = 0.037）。两组ET中SPA和巨噬细胞的表达不同。体外实验表明，巨噬细胞的迁移能力随SPA浓度的变化而变化（p< 0.05）。在用SPA处理巨噬细胞后进行RNA测序和蛋白质印迹。结论SPA可通过改变ET分泌物的表面张力，影响巨噬细胞的迁移，从而改变ET的功能。虽然OME这一领域的研究刚刚起步，但初步研究表明SPA可能在OME进展中起重要作用。证据水平NALryngoscope，133：1726-1733，2023
ObjectiveTo assess the role and possible mechanism of surfactant protein A (SPA) in the pathogenesis of otitis media with effusion (OME).MethodsThis was a multi‐part study with both an in vivo mouse model study as well as an in vitro study. The control and study groups (OME group) received phosphate‐buffered saline and inactivatedStreptococcus pneumoniae, respectively, via external auditory meatus injections. Changes in the surface tension of secretions from the eustachian tube (ET) and SPA expression were measured in both groups. A transwell assay was performed to observe the effect of different concentrations of SPA on the migration ability of macrophages. We examined the differentially expressed genes related to SPA‐treated macrophages using RNA‐seq analysis.ResultsOn Day 3, the surface tension of the OME group was higher than that of the control group (p= 0.014). The variation intensity of SPA in the ET of the OME group was significantly lower than that of the control group (p< 0.001). Surface tension was correlated with SPA (r= −0.525,p= 0.037). The expression of SPA and macrophages in the ET was different between the two groups. In vitro experiments revealed that macrophages showed different migration abilities with SPA concentration changes (p< 0.05). RNA‐seq and western blotting were performed after macrophages were treated with SPA. The results showed that RhoA and Rac1/2/3 were differentially expressed.ConclusionsSPA can change the surface tension of secretions from the ET and affect macrophage migration to alter the function of the ET. Although research in this field of OME is nascent, initial work suggests that SPA likely plays an important role in OME progression.Level of EvidenceNALaryngoscope, 133:1726–1733, 2023