Next-Generation Sequencing Approaches in Cancer: Where Have They Brought Us and Where Will They Take Us?
Next-Generation Sequencing Approaches in Cancer: Where Have They Brought Us and Where Will They Take Us?
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DOI：
10.3390/cancers7030869
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发表时间：
2015-09-23
期刊：
影响因子：
5.2
通讯作者：
Marra MA
中科院分区：
文献类型：
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作者：
LeBlanc VG;Marra MA
下一代测序（NGS）技术和数据已经彻底改变了癌症研究，并越来越多地被用于指导临床医生进行治疗决策。NGS技术使我们能够对癌症采取“组学”方法，以揭示个体恶性肿瘤的基因组、转录组和表观基因组景观。综合多平台分析越来越多地用于大规模项目，旨在全面表征个体肿瘤以及一般癌症类型和亚型。在这篇综述中，我们研究了NGS技术如何特别有助于癌症研究中的“组学”方法，允许考虑数百个肿瘤样本的大规模综合分析。这些类型的研究为我们提供了前所未有的丰富信息，提供了使小规模（包括“N of 1”）研究信息丰富和相关所需的背景知识。我们还将关注NGS和最先进的第三代测序技术提供的新兴机会，特别是在转化研究方面。癌症研究和护理目前正准备在可获得的测序技术的催化下取得重大进展，这将有利于临床和研究工作。
Next-generation sequencing (NGS) technologies and data have revolutionized cancer research and are increasingly being deployed to guide clinicians in treatment decision-making. NGS technologies have allowed us to take an “omics” approach to cancer in order to reveal genomic, transcriptomic, and epigenomic landscapes of individual malignancies. Integrative multi-platform analyses are increasingly used in large-scale projects that aim to fully characterize individual tumours as well as general cancer types and subtypes. In this review, we examine how NGS technologies in particular have contributed to “omics” approaches in cancer research, allowing for large-scale integrative analyses that consider hundreds of tumour samples. These types of studies have provided us with an unprecedented wealth of information, providing the background knowledge needed to make small-scale (including “N of 1”) studies informative and relevant. We also take a look at emerging opportunities provided by NGS and state-of-the-art third-generation sequencing technologies, particularly in the context of translational research. Cancer research and care are currently poised to experience significant progress catalyzed by accessible sequencing technologies that will benefit both clinical- and research-based efforts.