Regulated hyperacetylation of core histones during mouse spermatogenesis:: involvement of histone-deacetylases
Regulated hyperacetylation of core histones during mouse spermatogenesis:: involvement of histone-deacetylases
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DOI：
10.1078/0171-9335-00123
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发表时间：
2000-12-01
影响因子：
6.6
通讯作者：
Rousseaux, S
中科院分区：
文献类型：
--
作者：
Hazzouri, M;Pivot-Pajot, C;Rousseaux, S
在这里，我们报告了一个详细的分析波组蛋白乙酰化，发生在整个小鼠精子发生。我们的数据表明，精原细胞和preleptotene精母细胞含有乙酰化的核心组蛋白H2 A，H2 B和H4，而没有乙酰化组蛋白观察到整个减数分裂在细线期或粗线期精母细胞。组蛋白在大多数圆形精子细胞中保持未乙酰化，H2 A和H2 B，H3和H4的乙酰化形式在第9步中重新出现，以LI延长精子细胞，随后在凝聚精子细胞中消失。在精子细胞核内的乙酰化H4的空间分布模式，结合共聚焦显微镜，在3D免疫荧光分析，显示与染色质凝聚的事件的空间序列。为了深入了解精子发生过程中组蛋白乙酰化的控制机制，我们用组蛋白去乙酰化酶抑制剂，曲马斯他丁A（TSA）处理生精细胞，结果显示在圆形精子细胞中组蛋白乙酰化显著增加。这一观察结果表明，去乙酰化酶负责维持这些细胞中组蛋白的去乙酰化状态，TSA处理不能诱导凝聚精子细胞中组蛋白乙酰化，表明乙酰化的核心组蛋白被过渡蛋白取代而没有预先去乙酰化。此外，我们的数据显示凝聚精子细胞中组蛋白去乙酰化酶的显著减少，因此，组蛋白去乙酰化酶活性/浓度的调节似乎在控制精子发生过程中组蛋白过度乙酰化和可能的组蛋白替代中起主要作用。
Here we report a detailed analysis of waves of histone acetylation that occurs throughout spermatogenesis in mouse. Our data showed that spermatogonia and preleptotene spermatocytes contained acetylated core histones H2A, H2B and H4, whereas no acetylated histones were observed throughout meiosis in leptotene or pachytene spermatocytes. Histones remained unacetylated in most round spermatids, Acetylated forms of H2A and H2B, H3 and H4 reappeared in step 9 to LI elongating spermatids, and disappeared later in condensing spermatids. The spatial distribution pattern of acetylated H4 within the spermatids nuclei, analyzed in 3D by immunofluorescence combined with confocal microscopy; showed a spatial sequence of events tightly associated with chromatin condensation. In order to gain an insight into mechanisms controlling histone hyperacetylation during spermiogenesis, we treated spermatogenic cells with a histone deacetylase inhibitor, trichostatin A (TSA), which showed a spectacular increase of histone acetylation in round spermatids. This observation suggests that deacetylases are responsible for maintaining a deacetylated state of histones in these cells, TSA treatment could not induce histone acetylation in condensing spermatids, suggesting that acetylated core histones are replaced by transition proteins without being previously deacetylated, Moreover, our data showed a dramatic decrease in histone deacetylases in condensing spermatids, Therefore, the regulation of histone deacetylase activity/concentration appears to play a major role in controling histone hyperacetylation and probably histone replacement during spermiogenesis.