Comparison of ICAM-1 and VCAM-1 expression in various human endothelial cell types and smooth muscle cells
Comparison of ICAM-1 and VCAM-1 expression in various human endothelial cell types and smooth muscle cells
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DOI：
10.3109/10623329809053403
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发表时间：
1998-01-01
影响因子：
--
通讯作者：
Lelkes, PI
中科院分区：
文献类型：
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作者：
Kanda, K;Hayman, GT;Lelkes, PI
炎性血管病变局部表现的多样性可能部分归因于来自不同解剖位置的内皮细胞（EC）之间的异质性细胞粘附分子（CAM）表达。我们比较了基础和肿瘤坏死因子-α（TNF α，0-100 ng/ml，0-48 h）诱导的细胞间粘附分子-1（ICAM-1）和血管细胞粘附分子-1（VCAM-1）在培养的人主动脉EC（HAEC）、腔静脉EC（HVCEC）、真皮微血管EC（HMVEC）和腔静脉平滑肌细胞（HVCSM）中的表达，采用荧光ELISA和竞争性定量RT-PCR。我们发现，在蛋白质和mRNA水平上，基础ICAM-1表达存在显著差异，因此HAEC>HVCEC与HMVEC一致，远大于HVCSM。HVCEC中的基础VCAM-1 mRNA水平显着低于HAEC和HVCSM，而蛋白质水平则难以区分。所有EC中TNF α诱导的ICAM-1和VCAM-1水平相似，且显著高于HVCSM（分别为2.5倍和5倍）。血管细胞中基础和TNF α诱导的CAM表达水平不同可能解释了不同血管发生某些血管病变的不同倾向。
The diversity in the local manifestation of inflammatory vascular lesions might be partially attributable to heterogenous cell adhesion molecule (CAM) expression among endothelial cells (EC) derived from different anatomical locations. We compared basal and tumor necrosis factor-alpha (TNF alpha, 0-100 ng/ml, 0-48 h)-induced intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) expression in cultured human aortic EC (HAEC), vena cava EC (HVCEC), dermal microvascular EC (HMVEC), and vena cava smooth muscle cells (HVCSM), using a fluorescent ELISA and the competitive quantitative RT-PCR. We found marked differences in basal ICAM-1 expression, both at the protein and mRNA levels, such that HAEC>HVCEC congruent to HMVEC much greater than HVCSM. Basal VCAM-1 mRNA levels were significantly lower in HVCEC than in HAEC and HVCSM, while protein levels were indistinguishable. TNF alpha-induced ICAM-1 and VCAM-1 levels in all EC were similar and significantly higher than in HVCSM (2.5- and 5-fold, respectively). Dissimilar levels of basal and TNFa-induced CAM expression in vascular cells may explain the varied predisposition of different blood vessels to developing certain vasculopathies.