Cell suspensions of autologous keratinocytes or autologous fibroblasts accelerate the healing of full thickness skin wounds in a diabetic porcine wound healing model.
Cell suspensions of autologous keratinocytes or autologous fibroblasts accelerate the healing of full thickness skin wounds in a diabetic porcine wound healing model.
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DOI：
10.1016/j.jss.2008.10.001
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发表时间：
2009-11
期刊：
影响因子：
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通讯作者：
Patrik E. Velander;C. Theopold;O. Bleiziffer;J. Bergmann;H. Svensson;Yao Feng;E. Eriksson
中科院分区：
文献类型：
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作者：
Patrik E. Velander;C. Theopold;O. Bleiziffer;J. Bergmann;H. Svensson;Yao Feng;E. Eriksson
自体真皮成纤维细胞可用于治疗糖尿病皮肤伤口。我们假设培养的成纤维细胞或培养的角质形成细胞不仅能在高血糖伤口环境中存活，而且还能提高上皮再生的速率。我们之前开发了一种新的糖尿病猪皮肤伤口愈合延迟的猪模型。在背部创建全层伤口，并用聚氨酯腔室敷料以保持伤口湿润并允许监测伤口流体。将自体成纤维细胞或自体角质形成细胞的悬浮液注射到全层伤口中，并与在生理盐水中的潮湿环境中治疗的伤口进行比较。每天监测血清葡萄糖和伤口液葡萄糖浓度。监测伤口收缩，并在第12天进行活检。自体成纤维细胞或自体角质形成细胞悬液的移植增强了皮肤全层伤口的上皮再形成。用自体成纤维细胞治疗的伤口显示86.75%的再上皮化率，用自体角质形成细胞治疗的伤口显示91.3%的再上皮化率。这与在生理盐水处理的伤口中观察到的56.8%的上皮再形成率相比。虽然先前的研究已经显示成纤维细胞悬浮液在治疗非糖尿病伤口中的全层伤口中几乎没有效果，但是本研究显示在使用成纤维细胞或角质形成细胞悬浮液用于猪的糖尿病伤口的皮肤愈合中具有明显的有益效果。
Autologous dermal fibroblasts may be useful in the treatment of diabetic skin wounds. We hypothesized that cultured fibroblasts or cultured keratinocytes would not only survive in a hyperglycemic wound environment but also enhance the rate of re-epithelialization. We previously developed a new porcine model of delayed cutaneous wound healing in the diabetic pig. Full thickness wounds were created on the dorsum and dressed with polyurethane chambers to keep the wounds wet and to allow for wound fluid monitoring. Suspensions of either autologous fibroblasts or autologous keratinocytes were injected into full thickness wounds and compared with wounds treated in a wet environment in normal saline. Serum glucose and wound fluid glucose concentrations were monitored daily. Wound contraction was monitored and biopsies taken on day 12. Transplantation of suspensions of autologous fibroblasts or autologous keratinocytes enhanced re-epithelialization of cutaneous full thickness wounds. Wounds treated with autologous fibroblasts showed a re-epithelialization rate of 86.75% and wounds treated with autologous keratinocytes showed a re-epithelialization rate of 91.3%. This is compared with a re-epithelialization rate of 56.8% seen in the normal saline treated wounds. While previous studies have shown fibroblasts suspension to have little effect in the treatment of full thickness wounds in nondiabetic wounds, this study shows a clear beneficial effect in the use of fibroblast or keratinocyte suspensions for the cutaneous healing of diabetic wounds in pigs.