Susceptibility of different eukaryotic cell lines to SARS-coronavirus
Susceptibility of different eukaryotic cell lines to SARS-coronavirus
复制标题
DOI：
10.1007/s00705-004-0461-1
复制
发表时间：
2005-05-01
影响因子：
2.7
通讯作者：
Niedrig, M
中科院分区：
文献类型：
--
作者：
Hattermann, K;Müller, MA;Niedrig, M
为了确定和鉴定SARS冠状病毒（SARS-CoV）的靶细胞，我们研究了23种不同的永久性和原代真核细胞系对SARS冠状病毒的敏感性。在Vero E6细胞下，使用间接免疫荧光测定和新建立的定量实时PCR，也可以在两个猪细胞系（POEK，PS）和一个人细胞系（Huh-7）中证明SARS冠状病毒感染。RT-PCR检测到SARS-CoV感染的功能性受体血管紧张素转换酶2（ACE 2）的mRNA在所有敏感细胞系中表达。我们的研究结果表明，ACE 2 mRNA的丰度与SARS-CoV易感性之间存在相关性。
In order to define and characterize target cells of SARS-coronavirus (SARS-CoV) we studied the susceptibility of 23 different permanent and primary eukaryotic cell lines to SARS-coronavirus. Beneath Vero E6 cells SARS- Coronavirus infection could also be demonstrated in two pig cell lines (POEK, PS) and one human cell line (Huh-7) using the indirect immunofluorescence assay and a newly established quantitative real-time PCR. In all susceptible cell lines mRNA of the Angiotensin-converting enzyme 2 (ACE2), the functional receptor for SARS-CoV infection, could be detected by RT-PCR. Our results show that there is a correlation between the abundance of ACE2 mRNA and SARS-CoV susceptibility.