Sign-trackers have elevated myo-inositol in the nucleus accumbens and ventral hippocampus following Pavlovian conditioned approach.
Sign-trackers have elevated myo-inositol in the nucleus accumbens and ventral hippocampus following Pavlovian conditioned approach.
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DOI：
10.1111/jnc.13524
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发表时间：
2016-03
影响因子：
4.7
通讯作者：
Morrow JD
中科院分区：
文献类型：
--
作者：
Fitzpatrick CJ;Perrine SA;Ghoddoussi F;Galloway MP;Morrow JD
巴甫洛夫条件化方法(PCA)是一种行为学方法，可用于评估药物幼稚大鼠成瘾易感性的个体差异，并确定成瘾易感性因素。利用体外质子磁共振波谱(1H-MRS)，我们同时分析了PCA训练两周后中脑皮质边缘系统中多种神经化学物质的浓度，以确定药物幼稚大鼠可能的成瘾易感因素，为未来的研究做准备。与目标跟踪者或中间反应者相比，信号跟踪者伏隔核(NAC)和腹侧海马区(VHPC)的肌醇(INS)水平增加，但背侧海马区和前额叶内侧皮质的INS水平没有增加。此外，在NAC和vHPC，INS水平与PCA行为呈正相关。由于信号跟踪器的表型与更多的药物寻求行为有关，这些在药物幼稚大鼠身上观察到的结果表明，这些区域内胶质细胞活动/增殖的变化可能代表着成瘾易感性因素。
Pavlovian conditioned approach (PCA) is a behavioral procedure that can be used to assess individual differences in the addiction vulnerability of drug-naïve rats and identify addiction vulnerability factors. Using proton magnetic resonance spectroscopy (1H-MRS) ex vivo, we simultaneously analyzed concentrations of multiple neurochemicals throughout the mesocorticolimbic system two weeks after PCA training in order to identify potential vulnerability factors to addiction in drug naïve rats for future investigations. Levels of myo-inositol (Ins), a 1H-MRS-detectable marker of glial activity/proliferation, were increased in the nucleus accumbens (NAc) and ventral hippocampus (vHPC), but not dorsal hippocampus or medial prefrontal cortex, of sign-trackers compared to goal-trackers or intermediate responders. In addition, Ins levels positively correlated with PCA behavior in the NAc and vHPC. Because the sign-tracker phenotype is associated with increased drug-seeking behavior, these results observed in drug-naïve rats suggest that alterations in glial activity/proliferation within these regions may represent an addiction vulnerability factor.