Rubella epidemic in Vietnam: Characteristic of rubella virus genes from pregnant women and their fetuses/newborns with congenital rubella syndrome
Rubella epidemic in Vietnam: Characteristic of rubella virus genes from pregnant women and their fetuses/newborns with congenital rubella syndrome
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DOI：
10.1016/j.jcv.2013.02.008
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发表时间：
2013-06-01
影响因子：
8.8
通讯作者：
Abe, Kenji
中科院分区：
文献类型：
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作者：
Van Hung Pham;Thong Van Nguyen;Abe, Kenji
背景资料：风疹在东南亚地区的控制仍然很差，包括Vietnam. Objectives：本研究的目的是描述2011 - 2012年期间风疹病毒在越南的传播情况。研究设计：从130例患者（110例疑似风疹的孕妇和20例胎儿/新生儿）中收集羊水、咽拭子和胎盘样本。直接从临床标本中提取病毒RNA，经PCR扩增后，对WHO推荐的用于鉴定病毒基因型的E1基因（含739个核苷酸）进行测序。通过实时PCR筛选，110例羊水中有103例可检测到病毒RNA（93.6%）疑似风疹的孕妇和所有20例（100%）胎儿/新生儿的咽拭子中检出风疹。此外，还在所有胎儿/新生儿病例的胎盘中检测到病毒RNA。20例胎儿/新生儿均表现为先天性白内障。通过PCR扩增获得24株E1基因阳性菌株。使用风疹参考序列的系统发育分析，所有的菌株被发现是基因型2B。有趣的是，94%（30/32）的越南株，包括9株从数据库中，形成了一个独立的集群内的基因型2B表明土著病毒在该region.Conclusions流行：风疹病毒在越南确定属于基因型2B。重要的是，胎儿/新生儿风疹病毒感染率为100%，所有人都患有先天性白内障。我们的研究结果表明，在该地区建立风疹预防是一项紧迫的任务，以改善孕产妇和儿童健康。(C)2013 Elsevier B.V.保留所有权利。
Background: Rubella remains poorly controlled in Southeast Asia, including Vietnam.Objectives: The aim of this study was to characterize rubella virus spread in Vietnam during 2011-2012.Study design: Amniotic fluid, throat swab and placenta samples were collected from 130 patients (110 cases from pregnant women with suspected rubella and 20 cases from fetuses/newborns). Viral RNA was obtained directly from clinical specimens, amplified by PCR, and then the E1 gene containing 739 nucleotides recommended by the WHO to identify the viral genotypes was sequenced.Results: By screening with real-time PCR, viral RNA was detectable in amniotic fluids from 103 out of 110 (93.6%) pregnant women with suspected rubella and in the throat swabs from all of 20 (100%) fetuses/newborns. In addition, viral RNA was also detected in the placenta from all cases of fetuses/newborns. All of 20 fetuses/newborns presented with congenital cataract. Twenty-four strains with the E1 gene were obtained by PCR. Using phylogenetic analysis with rubella reference sequences, all of the strains were found to be genotype 2B. Interestingly, 94% (30/32) of Vietnamese strains, including 9 strains from the database, formed an independent cluster within the genotype 2B suggesting that indigenous viruses are prevalent in this region.Conclusions: Rubella virus identified in Vietnam belonged to the genotype 2B. Importantly, the infection rate of rubella virus in fetuses/newborns was 100% and all of them had congenital cataract. Our results indicate an establishment of rubella prevention in this area is an urgent task in order to improve maternal and child health. (C) 2013 Elsevier B.V. All rights reserved.