Selective sterol-phospholipid associations in fluid bilayers.
Selective sterol-phospholipid associations in fluid bilayers.
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流体双层中的选择性甾醇-磷脂结合。
DOI：
10.1021/ja017269i
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发表时间：
2002
影响因子：
15
通讯作者：
Regen,StevenL
中科院分区：
文献类型：
--
作者：
Sugahara,Michihiro;Uragami,Maki;Regen,StevenL
三种可交换的脂质单体（两种长度不同的磷脂A和B，以及胆固醇衍生物C）的最近邻优先性已经通过使用最近邻识别方法在流体双层状态下定量（Davidson，S. K. M.; Regen，S. L.Chem.Rev.1997，97，1269）。因此，对平衡二聚体分布的分析已经表明：（i）固醇相对于其他固醇分子而言，倾向于将两种磷脂作为最近邻，（ii）这种识别是选择性的（即，甾醇倾向于较长的磷脂而不是较短的磷脂作为最近邻，特别是当双层中甾醇浓度高时（例如，40摩尔%），和（iii）磷脂本身不能相互识别。综上所述，这些发现表明胆固醇诱导A和Bin类似双层的同源结合的可能机制是固醇从随机混合的A和B的“海洋”中“拉”出两个或更多的较长的磷脂单体（B）。这些发现也支持了流体双层中胆固醇-磷脂复合物的概念。这些研究结果的生物学意义进行了简要讨论。
The nearest-neighbor preferences of three exchangeable lipid monomers (two phospholipids that differ in length,AandB, and a derivative of cholesterol,C) have been quantified in the fluid bilayer state by use of the nearest-neighbor recognition method (Davidson, S. K. M.; Regen, S. L.Chem. Rev.1997,97, 1269). Thus, an analysis of the equilibrium dimer distributions has shown that (i) the sterol favors both phospholipids as nearest neighbors relative to other sterol molecules, (ii) that this recognition is selective (i.e., the sterol favors the longer phospholipid as a nearest neighbor over the shorter one, especially when the sterol concentrations in the bilayer is high (e.g., 40 mol %), and (iii) the phospholipids, themselves, are unable to recognize each other. Taken together, these findings indicate that the probable mechanism by which cholesterol induces homoassociation ofAandBin analogous bilayers is one in which the sterol “pulls” two or more of the longer phospholipid monomers (B) out of a “sea” of randomly mixedAandB. These findings also lend support for the notion of cholesterol−phospholipid complexation in fluid bilayers. The biological implications of these findings are briefly discussed.