C/ATF, A MEMBER OF THE ACTIVATING TRANSCRIPTION FACTOR FAMILY OF DNA-BINDING PROTEINS, DIMERIZES WITH CAAT ENHANCER-BINDING PROTEINS AND DIRECTS THEIR BINDING TO CAMP RESPONSE ELEMENTS
C/ATF, A MEMBER OF THE ACTIVATING TRANSCRIPTION FACTOR FAMILY OF DNA-BINDING PROTEINS, DIMERIZES WITH CAAT ENHANCER-BINDING PROTEINS AND DIRECTS THEIR BINDING TO CAMP RESPONSE ELEMENTS
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DOI：
10.1073/pnas.90.10.4679
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发表时间：
1993-05-15
影响因子：
11.1
通讯作者：
HABENER, JF
中科院分区：
文献类型：
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作者：
VALLEJO, M;RON, D;HABENER, JF
碱性亮氨酸拉链(BZip)转录因子C/EBP家族的成员形成异源二聚体，并与CAAT盒和其他序列相关的增强子基序结合。利用P-32标记的蛋白探针，我们克隆了一个编码C/EBP相关ATF(C/ATF)的克隆。C/ATF是一种与C/EBP样蛋白异构化的bZip蛋白，属于CREB/ATF家族。C/ATF同源二聚体不与典型的C/EBP DNA位点结合。相反，它们与回文cAMP反应元件结合，例如生长抑素基因。此外，C/ATF-C/EBPbeta异源二聚体与不对称cAMP反应元件的亚类结合，磷酸烯醇式丙酮酸羧激酶和前脑啡肽基因中的反应元件就是例证。瞬时转基因研究表明，C/ATF和C/EBPβ之间的相互作用是这两个转录因子家族之间功能交叉的基础，这可能对激素和发育刺激的整合至关重要，这些刺激决定了特定细胞表型中基因亚组的表达。
Members of the C/EBP family of basic-leucine zipper (bZip) transcription factors form heterodimers and bind to the CAAT box and other sequence-related enhancer motifs. Using a P-32-labeled protein probe consisting of the bZip domain of C/EBPbeta, we isolated a clone encoding C/EBP-related ATF (C/ATF), a bZip protein that heterodimerizes with C/EBP-like proteins but belongs to the CREB/ATF family. C/ATF homodimers do not bind to typical C/EBP DNA sites. Instead they bind to palindromic cAMP response elements such as that of the somatostatin gene. In addition, C/ATF-C/EBPbeta heterodimers bind to a subclass of asymmetric cAMP response elements exemplified by those in the phosphoenolpyruvate carboxykinase and proenkephalin genes. Transient transfection studies indicate that interactions between C/ATF and C/EBPbeta are the basis for a functional cross talk between these two families of transcription factors that may be important for the integration of hormonal and developmental stimuli that determine the expression of subsets of genes in specific cellular phenotypes.