Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits.
Distribution of ESCRT machinery at HIV assembly sites reveals virus scaffolding of ESCRT subunits.
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艾滋病毒组装部位的ESCRT机械的分布揭示了ESCRT亚基的病毒脚手架。
DOI：
10.1126/science.1247786
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发表时间：
2014-02-07
期刊：
影响因子：
--
通讯作者：
Lippincott-Schwartz J
中科院分区：
文献类型：
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作者：
Van Engelenburg SB;Shtengel G;Sengupta P;Waki K;Jarnik M;Ablan SD;Freed EO;Hess HF;Lippincott-Schwartz J
人类免疫缺陷病毒(HIV)劫持运输所需的内体分选复合体(ESCRT)，以介导病毒从感染细胞释放。介导病毒脱落所需的ESCRT机制的纳米级组织尚不清楚。在这里，我们应用三维超分辨率显微镜和相关的电子显微镜来描绘在HIV组装位置的ESCRT组件的组织。我们观察到ESCRT亚基定位于萌芽中的病毒粒子和释放的颗粒的头部，当病毒脱落时，头部定位的CHMP2A水平相对于Tsg101和CHMP4B下降。因此，HIV释放的驱动力可能来自于病毒芽内部ESCRT亚基的初始支架，随后是质膜结合和ESCRT亚基的选择性重塑。
The human immunodeficiency virus (HIV) hijacks the endosomal sorting complexes required for transport (ESCRT) to mediate virus release from infected cells. The nanoscale organization of ESCRT machinery necessary for mediating viral abscission is unclear. Here, we apply three-dimensional superresolution microscopy and correlative electron microscopy to delineate the organization of ESCRT components at HIV assembly sites. We observe ESCRT subunits localize within the head of budding virions and released particles, with head-localized levels of CHMP2A decreasing relative to Tsg101 and CHMP4B upon virus abscission. Thus, the driving force for HIV release may derive from initial scaffolding of ESCRT subunits within the viral bud interior followed by plasma membrane association and selective remodeling of ESCRT subunits.