Effects of IL-1β, IL-6 and IL-8 on erythrocytes, platelets and clot viscoelasticity.
Effects of IL-1β, IL-6 and IL-8 on erythrocytes, platelets and clot viscoelasticity.
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DOI：
10.1038/srep32188
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发表时间：
2016-08-26
影响因子：
4.6
通讯作者：
Pretorius E
中科院分区：
文献类型：
--
作者：
Bester J;Pretorius E
细胞因子之间存在复杂的相互作用，白细胞介素家族在炎症中发挥着重要作用。特别是循环中的 IL-1β、IL-6 和 IL-8 在全身和慢性炎症条件下不受调节。高凝状态是炎症的重要标志，这些细胞因子与异常凝块形成、红细胞病理学和血小板过度活化密切相关，并且这三种细胞因子在血小板上具有已知的受体。尽管这些细胞因子在炎症中总是不受调节，但我们不知道单个细胞因子如何作用于红细胞和血小板的结构，以及哪些粘弹性血块参数发生变化。在这里，我们通过使用扫描电子显微镜和血栓弹力图研究低生理水平（代表慢性炎症）的 IL-1β、IL-6 和 IL-8 的影响。所有三种白细胞介素均导致粘弹性特性显示全血的高凝性以及红细胞和血小板的病理学增加。最显着的变化是所有三种细胞因子均导致血小板过度激活和扩散。 IL-8 存在时，红细胞结构受到显着影响，其形态变化类似于红细胞凋亡（程序性细胞死亡）中常见的情况。我们认为红细胞和血小板对细胞因子的存在特别敏感，并且它们是极好的健康指标。
Complex interactions exist between cytokines, and the interleukin family plays a fundamental role in inflammation. Particularly circulating IL-1β, IL-6 and IL-8 are unregulated in systemic and chronic inflammatory conditions. Hypercoagulability is an important hallmark of inflammation, and these cytokines are critically involved in abnormal clot formation, erythrocyte pathology and platelet hyper-activation, and these three cytokines have known receptors on platelets. Although these cytokines are always unregulated in inflammation, we do not know how the individual cytokines act upon the structure of erythrocytes and platelets, and which of the viscoelastic clot parameters are changed. Here we study the effects of IL-1β, IL-6 and IL-8 at low physiological levels, representative of chronic inflammation, by using scanning electron microscopy and thromboelastography. All three interleukins caused the viscoelastic properties to display an increased hypercoagulability of whole blood and pathology of both erythrocytes and platelets. The most pronounced changes were noted where all three cytokines caused platelet hyper-activation and spreading. Erythrocyte structure was notably affected in the presence of IL-8, where the morphological changes resembled that typically seen in eryptosis (programmed cell death). We suggest that erythrocytes and platelets are particularly sensitive to cytokine presence, and that they are excellent health indicators.