Neurogenesis in the adult human hippocampus
Neurogenesis in the adult human hippocampus
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DOI：
10.1038/3305
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发表时间：
1998-11-01
期刊：
影响因子：
82.9
通讯作者：
Gage, FH
中科院分区：
文献类型：
--
作者：
Eriksson, PS;Perfilieva, E;Gage, FH
在成年人类大脑中，包括神经元在内的新细胞的产生尚未得到证实。本研究旨在调查在成年人类大脑中，在先前已确定在成年啮齿动物和猴子中具有神经发生能力的区域是否会发生神经发生。人类脑组织取自死后接受过胸苷类似物溴脱氧尿苷（BrdU）治疗的患者，BrdU可在S期标记DNA。通过对BrdU以及神经元标志物之一（NeuN、钙结合蛋白或神经元特异性烯醇化酶（NSE））进行免疫荧光标记，我们证明了由这些标志物所定义的新神经元是由成年人类齿状回中的分裂祖细胞产生的。我们的研究结果进一步表明，人类海马体在整个生命过程中都保留着产生神经元的能力。
The genesis of new cells, including neurons, in the adult human brain has not yet been demonstrated. This study was undertaken to investigate whether neurogenesis occurs in the adult human brain, in regions previously identified as neurogenic in adult rodents and monkeys. Human brain tissue was obtained postmortem from patients who had been treated with the thymidine analog, bromodeoxyuridine (BrdU), that labels DNA during the S phase. Using immunofluorescent labeling for BrdU and for one of the neuronal markers, NeuN, calbindin or neuron specific enolase (NSE), we demonstrate that new neurons, as defined by these markers, are generated from dividing progenitor cells in the dentate gyrus of adult humans. Our results further indicate that the human hippocampus retains its ability to generate neurons throughout life.