Transplanted adult spinal cord-derived neural stem/progenitor cells promote early functional recovery after rat spinal cord injury
Transplanted adult spinal cord-derived neural stem/progenitor cells promote early functional recovery after rat spinal cord injury
复制标题
DOI：
10.1016/j.neuroscience.2008.05.042
复制
发表时间：
2008-08-26
期刊：
影响因子：
3.3
通讯作者：
Tator, C. H.
中科院分区：
文献类型：
--
作者：
Parr, A. M.;Kulbatski, I.;Tator, C. H.
我们检测了脊髓源性神经干/祖细胞(NSPC)在延迟移植到损伤的成年大鼠脊髓后，在早期或早期移植骨髓间充质干细胞(BMSCs)的效果。夹伤后即刻移植骨髓间充质干细胞或培养液(27g力)，伤后9d移植神经干细胞或培养液。评估细胞存活和分化、功能恢复、轴突逆行追踪和免疫电子显微镜检查。基于三种不同措施的功能恢复仅在接受非骨髓间充质干细胞移植的NSPC组中显著改善，且在移植后1周内恢复明显。在这组中，NSPC主要分化为少突胶质细胞和星形胶质细胞，移植的NSPC在损伤部位有轴突内鞘，宿主少突胶质细胞增加，逆行标记的脊髓上核增加的趋势。移植骨髓间充质干细胞支架后，神经干细胞的存活率有提高的趋势，但功能没有增加。因此，成年大鼠移植NSPC后，脊髓损伤后早期功能有显著改善，提示移植的NSPC具有与少突胶质细胞存活和轴突包裹相关的早期神经保护作用。(C)2008年IBRO。爱思唯尔有限公司出版。保留所有权利。
We examined the effect of spinal cord-derived neural stem/progenitor cells (NSPCs) after delayed transplantation into the injured adult rat spinal cord with or without earlier transplantation of bone marrow-derived mesenchymal stromal cells (BMSCs). Either BMSCs or culture medium were transplanted immediately after clip compression injury (27 g force), and then, 9 days after injury, NSPCs or culture medium were transplanted. Cell survival and differentiation, functional recovery, retrograde axonal tracing, and immunoelectron microscopy were assessed. A significant improvement in functional recovery based on three different measures was seen only in the group receiving NSPCs without BMSCs, and the improved recovery was evident within 1 week of transplantation. In this group, NSPCs differentiated mainly into oligodendrocytes and astrocytes, there was ensheathing of axons at the injury site by transplanted NSPCs, an increase in host oligodendrocytes, and a trend toward an increase in retrogradely labeled supraspinal nuclei. Transplantation of the BMSC scaffold resulted in a trend toward improved survival of the NSPCs, but there was no increase in function. Thus, transplantation of adult rat NSPCs produced significant early functional improvement after spinal cord injury, suggesting an early neuroprotective action associated with oligodendrocyte survival and axonal ensheathment by transplanted NSPCs. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.